FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872771
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23600.036.6234861
GGTATCA10700.032.6954651
TATCAAC27500.031.4296112
ATCAACG27400.031.4191383
CAACGCA27950.030.8008755
AACGCAG28150.030.5837926
TCAACGC28150.030.5820394
GTGGTAT4950.029.794381
AGAGTAC30150.028.21210711
ACGCAGA30750.027.996247
CGCAGAG31250.027.54838
CAGAGTA31850.026.81396510
GCAGAGT33650.025.5834889
TGGTATC5900.025.5783062
ACGAGAC1801.4551915E-1124.7765922
TACGAGT700.001379962824.4987034
GTGTAAG1653.3614924E-922.8654581
GAGTACG27400.021.65542212
AGTACGG27150.021.60093313
CGAGACT2251.2732926E-1121.3484323
Produced by FastQC (version 0.11.5)