FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872771
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11520.1319933865813598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23450.034.1181341
CAATCGG1002.5522432E-432.6895270
CGTTAAT450.004729879630.522471
TATCAAC26950.029.7992362
GGTATCA11750.029.2236421
ATCAACG27800.028.379653
TCAACGC28200.027.8506764
AACGCAG28000.027.6837336
CAACGCA28600.027.3428045
GTGGTAT6150.026.2418791
ACGCAGA30050.025.6810237
AGAGTAC30300.024.90315211
CGCAGAG31150.024.7741478
CAGAGTA32000.024.00890210
CCTATAC1301.9516665E-723.7477873
GCAGAGT33100.023.6255049
TGGTATC6900.022.3826482
GTTAGAC800.002997128821.4389763
GCTAACC800.002997128821.4389763
GTACACG1154.771133E-520.9012571
Produced by FastQC (version 0.11.5)