FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966506
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29380.30398155831417495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26350.042.0889851
TATCAAC29700.037.0937772
ATCAACG29750.036.7861943
TCAACGC29900.036.708434
CAACGCA30650.035.810185
GGTATCA9850.034.751041
AACGCAG31500.034.7353256
ACGCAGA33400.032.6569947
CAGAGTA34450.031.7592510
CGCAGAG34500.031.6157538
AGAGTAC35050.031.02048511
CGAATTA450.004826740430.39655715
GTGGTAT3950.029.4636041
GCAGAGT37350.029.2033069
CGCGACT500.007126050528.06703653
AGTACGG27550.027.5512213
GAGTACG27350.027.50266812
GTACGGG31850.023.8315914
TACGGGC6500.023.6742415
TGGTATC5250.023.460912
Produced by FastQC (version 0.11.5)