FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68360
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1210.1770040959625512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCT150.002502905267.8829624
CGCCCGT200.00733191651.74498438
TCGCCCG200.007396859651.62982637
AGGCCAT200.007795555550.9494910
GCAGTGC200.00781816450.9122223
AATTGAG200.00781816450.9122220
GTATCAA2150.045.781531
TATCAAC2300.042.7957762
ATCAACG2400.041.012623
CAACGCA2350.039.0251435
AACGCAG2350.039.0251436
TCAACGC2400.038.212124
CGCAGAG2750.033.3487558
GGTATCA652.5602158E-531.3305951
AGAGTAC3100.030.65681811
AGTACTT1800.030.17020213
ACGCAGA3100.029.5835727
CAGAGTA3100.029.58357210
TACGGGG854.937523E-627.95180515
GAGTACT1950.027.84941712
Produced by FastQC (version 0.11.5)