FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68360
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5910.8645406670567584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACA200.00695170452.44549643
GGTTACC200.00789140150.79215626
GTATCAA1850.042.0980031
TGGACCT350.001483658838.6138574
TATCAAC2200.035.3487632
ATCAACG2150.034.5728723
GCTCTAG400.002826749833.8614351
GGTATCA701.1062657E-633.8614351
TCAACGC2200.033.787134
AACGCAG2250.033.03636
CAACGCA2300.032.318125
ACACAAC500.007066216328.07720445
CGCAGAG2650.028.0496888
CACACAA500.00711887628.03459744
CTGTACT500.00850907227.02974
GCAGAGT2650.026.7747029
ACGCAGA2800.026.5470267
AGAGTAC2800.025.34034311
GAGTACG951.2134393E-524.89577712
AGTACGG951.2134393E-524.89577713
Produced by FastQC (version 0.11.5)