FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532780
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG1200.042.85704828
ACGCGTA1150.041.7272727
GTATCAA15750.038.6754761
CGTATGC901.1823431E-1038.4823741
TCGTATG901.1823431E-1038.4385540
GGTATCA7250.038.233431
GCGTAGT1400.036.75535629
CTCGTAT952.1464075E-1036.37750239
GACGCGT1400.034.2566526
ACGAGAC1400.034.23734722
TATCTCG400.002700387534.221284
CTTATAC1550.033.1173671
TATCAAC18550.032.837672
TAGTAAT1700.032.33268732
TCAACGC18900.032.2295654
ATCAACG19050.032.1554263
CAACGCA19550.030.9829465
CGTATGA450.004431232330.92986344
TATACAC1900.030.619043
AACGCAG19950.030.1930356
Produced by FastQC (version 0.11.5)