FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532780
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11210.21040579601336387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17350.037.3027081
TATCAAC19250.033.579862
ATCAACG19300.032.9581383
GTGGTAT3400.032.229171
AACGCAG19750.032.207196
TCAACGC19750.032.207194
CAACGCA20100.031.646375
GGTATCA7900.031.642721
ACGCAGA21600.029.6070337
CCCTTAG704.048119E-529.3515641
CGCAGAG22250.028.742118
AGAGTAC21450.028.53860711
ACGGGTA605.697616E-428.504116
CAGAGTA21750.028.30220410
GTAACAC854.7348694E-628.1634713
GCAGAGT23550.027.1554979
TGGTATC3650.026.2369272
AGAGTCG951.1233091E-525.19889311
CGAATTA851.5284057E-424.1446515
GAGTACT9100.023.30007612
Produced by FastQC (version 0.11.5)