FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086455
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54580.26159203050149654No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT41890.20077116448713248No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36800.176375718623215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35500.1701450546501123No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34710.16635872808184216No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34560.16563980531571493No Hit
CTATGATACTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC33970.16281204243561448No Hit
GTATCATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTT30490.14613303426146262No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG30150.14450347599157423No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA29210.1399982266571769No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29170.13980651391954294No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28850.13827281201847152No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28830.13817695564965457No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACAGTATCATAGTTATTGGACGTTCGG28240.1353491927695541No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA28190.1351095518475117No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCTTCAGGGAGA28030.13434270089697598No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26440.12672211957602728No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26160.12538013041258977No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA26060.12490084856850495No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25350.12149794747550272No Hit
GTGCAGCCACAGTTCGTCTGATATCCACCTTGGTCCCTTGGCCGAACGTC25130.12044352741851609No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23010.11028275232391783No Hit
GTCTATTACTGTGCGAGGGGGGGATATTGTACTACTACCAACTGCTATATGGGTCTTCGGAGTGACTACTGGTA22160.10620885664919684No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21920.10505858022339326No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21850.1047230829325339No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21540.10323730921587093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47000.042.077691
TATCAAC53650.037.1817442
ATCAACG54650.036.6272473
TCAACGC55350.036.0397574
CAACGCA56400.035.4297835
AACGCAG57450.034.842116
TACTGAA14200.033.28022470
GTGGTAT6050.032.97271
ACGCAGA64250.031.0987727
CGCAGAG64300.031.0745898
AGAGTAC64400.030.91953711
TAGACCG703.9349317E-529.479815
GCAGAGT68100.029.239629
GTCTACT10450.026.83817967
AGTACGG42950.026.42354213
GAGTACG43350.026.33839212
CTACTGA12900.025.75540769
GTGTCTA11500.025.51317665
GACGTGT11100.025.31752662
CGTGTCT11200.025.16107664
Produced by FastQC (version 0.11.5)