FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086455
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56170.2692126118224452No Hit
CTATGATACTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC39010.18696784737748956No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT35460.16995334191247835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32760.1570127321221881No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32650.1564855220936948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32140.15404118468886221No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31710.15198027275929746No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31520.1510696372555363No Hit
GTATCATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTT31170.1493921508012394No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30600.14666024428995592No Hit
GTGCAGCCACAGTTCGTCTGATATCCACCTTGGTCCCTTGGCCGAACGTC27220.13046051795988892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25760.12346300303625049No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACAGTATCATAGTTATTGGACGTTCGG25580.12260029571689782No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCTTCAGGGAGA25350.12149794747550272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25300.12125830655346029No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA25290.12121037836905181No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA24170.11584242171530179No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG22850.10951590137338212No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22770.10913247589811427No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA22660.10860526586962096No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22500.10783841491908523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22170.10625678483360533No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21870.10481893930135086No Hit
GTCTATTACTGTGCGAGGGGGGGATATTGTACTACTACCAACTGCTATATGGGTCTTCGGAGTGACTACTGGTA20920.10026576178254504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48050.039.796221
GTGGTAT6900.036.8844261
TATCAAC54250.035.1568372
TCAACGC57250.033.5337564
ATCAACG57850.033.2509043
CAACGCA58350.032.960445
AACGCAG59750.032.4755446
CGCAGAG65650.029.713898
AGAGTAC65900.029.1835511
ACGCAGA67050.029.0934647
GCAGAGT69750.027.868799
TTCGGGG11800.027.54057370
TACTGAA11300.025.2376570
AGTACGG49350.024.98289513
GAGTACG50250.024.60378512
TTAACGG1554.087633E-822.25958835
GAGTACT31200.022.12467812
AGTACTT31300.021.9442713
TGTGCGA8600.021.56407410
TGTTAGA11600.020.5827570
Produced by FastQC (version 0.11.5)