FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785406
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47630.2667740558730059No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTCTC40800.2285194515981239No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGAGAGAATTCA35520.1989463460971902No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35510.19889033642768086No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35500.19883432675817156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31140.17441411085209751No Hit
GCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGC30740.17217372407172374No Hit
GCTTAATAGTTACCCTATCACCTTCGGCCAAGGGACACGACTGGAGATTAAGCGAACTGTGGCTGCACCAT30720.17206170473270505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29250.16382828331483146No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28380.15895544206751855No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28100.1573871713212569No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27760.1554828425579392No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTAAGAGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAGA27740.1553708232189205No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26810.15016192395455152No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26140.14640927609742546No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT26140.14640927609742546No Hit
GTGCAGCCACAGTTCGCTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG25890.14500903435969184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23980.13431118748340715No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22770.1275340174727765No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22590.1265258434216083No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22000.123221272920557No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT21410.11991670241950571No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20520.11493184183317409No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19840.11112318430653868No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19460.10899481686518361No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19030.10658640107628181No Hit
GACCAGGGAAAGCCCCTCAGCTCCTGATCTATGCTGCGTCCACTTTGCAA18590.10412197561787066No Hit
GTGATAGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTC18320.10260971454111839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)