FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883374
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT102840.35666548980465246No Hit
GATCTATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCA88030.305302052387238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG68720.23833189867148694No Hit
GTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTT66210.2296268191361925No Hit
GAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAACTGTGGCTGCACCAT65110.2258118440410436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62520.2168293117715565No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61050.21173111778076656No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT60640.21030917251802922No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG59510.20639015264755806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT59330.20576588399562457No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC58420.20260985914418317No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56320.1953267248716261No Hit
ATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCACTCTCA54990.19071407316567326No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53760.18644823737746125No Hit
GCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGG53740.1863788741939131No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53310.1848875657476276No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACT52660.1826332622823123No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC51880.17992809812393395No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG51850.17982405334861173No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT51220.1776391130668446No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA50570.17538480960152933No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAA50560.17535012800975525No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC49780.1726449638513769No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47370.1642867002338233No Hit
AGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATA47130.16345434203124534No Hit
GTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACA45610.15818274008158498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45470.15769719779674785No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA45210.15679547641062172No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT44600.15467989931240275No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42040.14580141181823794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40880.1417783471724445No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAA39950.13855295913745494No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT39870.13827550640326228No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT39050.13543161587778763No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT38120.13220622784279804No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGGGGTA37000.12832188956410095No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATTTACTGCCA36800.12762825772861933No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35210.12211388463654038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35130.12183643190234773No Hit
GTAATACACGGCCGTGTCCTCAGATCCCAGGCTGCTCAGCTCCATGTAGGCTGTACTTATGGAGGTGTTCCAGGT35010.12142025280105877No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33790.11718909860462083No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33270.11538565583236861No Hit
GTGTATTACTGTGCGAGAGAACAACTGTATTGTGCTCATGGTGTATGCTA32820.11382498420253494No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32740.11354753146834229No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32180.11160536232899373No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC32080.11125854641125293No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA31380.10883083498706725No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAA31090.10782506882561887No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31010.10754761609142623No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT30680.10640312356288155No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30550.10595226286981849No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTT30150.10456499919885523No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC30020.10411413850579217No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30010.1040794569140181No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC29990.10401009373046993No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT29320.10168642708160648No Hit
CTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGA29200.10127024798031749No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29100.1009234320625767No Hit
ATGCAGTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTTAAATAGTTGGCAATGCT29020.10064597932838404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7300.040.987171
GTATCAA53800.039.6972921
TATCAAC59800.035.7149052
ATCAACG63150.033.92983
TCAACGC63450.033.8241654
CAACGCA64850.033.2536475
AACGCAG65950.032.7517176
CGTATGC2750.031.45925341
TGACTTG25850.030.96395770
CGCAGAG72250.029.89438
AGAGTAC72300.029.68128811
ACGCAGA77100.028.05847
CAGAGTA79200.027.18273710
TTCGGGG26550.026.6594370
GAGTACT33600.025.58760812
AGTACTT34100.025.31283413
GAGCAAA34950.025.17306570
CGGGACT6450.024.528574
TATGCCG3650.023.72412143
CACCAGT29400.023.17509770
Produced by FastQC (version 0.11.5)