FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883374
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT102160.35430714156401494No Hit
GATCTATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCA85180.2954177987316248No Hit
GTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTT66140.22938404799377393No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT63730.22102578437622036No Hit
GAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAACTGTGGCTGCACCAT61580.2135692421447929No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG61330.21270220235044082No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG59720.20711846607481374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59090.20493352579304663No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58570.20313008302079438No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT58240.20198559049224968No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC55770.1934192373240516No Hit
ATACTGCATTCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAAATCTGGGACAGATTTCACTCTCA52880.18339625730134212No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA51600.1789570135542597No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50710.17587035188636646No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAA50050.17358136682927708No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAA48930.16969702855058No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48770.16914212308219467No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACT48230.16726931712639428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47180.16362774999011573No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC47120.16341966043947126No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47100.1633502972559231No Hit
GCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGG46090.15984745648674087No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT44720.15509607841369175No Hit
AGTCAGAGCATTGCCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATA44670.15492267045482133No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA44000.15259900380595787No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG43930.15235623266353931No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC43910.15228686947999115No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43810.15194005356225032No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42940.14892275507790526No Hit
GTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACA41930.14541991430872303No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT41740.14476096406501548No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT41610.14431010337195244No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT40240.13955872529890329No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39280.1362292924885915No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37300.12936233731732338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA35970.12474968561137056No Hit
GTAATACACGGCCGTGTCCTCAGATCCCAGGCTGCTCAGCTCCATGTAGGCTGTACTTATGGAGGTGTTCCAGGT35820.12422946173475935No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT35600.12346646671572957No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATTTACTGCCA34500.11965149162058061No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34160.11847231750026184No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATATCGACTTTTGTCCCAGGGCCGAAAGTTCGAGGGGTA33970.11781336725655431No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31710.10997532751561193No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31030.10761697927497439No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30770.10671525788884828No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30360.10529331262611094No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30240.10487713352482196No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTTCGGCCCTGGGACAAAAGTCGATATCACACGAA30180.10466904397417748No Hit
GTGTATTACTGTGCGAGAGAACAACTGTATTGTGCTCATGGTGTATGCTA29870.10359391462918094No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCGAACTTT29750.10317773552789197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29560.10251878528418443No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29540.10244942210063626No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29500.10231069573353996No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC29360.1018251534487028No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG29240.10140897434741383No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA28960.10043788977773956No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT28860.10009107385999874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7700.042.880671
GTATCAA53000.041.597121
TATCAAC60600.036.185552
TCAACGC62100.035.1824954
ATCAACG62450.035.0977673
CAACGCA64350.034.0596965
AACGCAG65550.033.59346
CGCAGAG71700.030.8077858
AGAGTAC72750.030.78705811
ACGCAGA76000.028.974317
CAGAGTA79000.028.43932510
TTACGCG500.00787465727.50029823
TTCGGGG24150.026.95020970
TGACTTG23450.025.44180370
AGTACGG59350.024.48305913
CCTATAC1551.6043487E-924.381043
GAGTACT32200.024.11007912
GAGTACG60700.024.05172712
GCAGAGT95000.023.5410619
AGTACTT33150.023.4191413
Produced by FastQC (version 0.11.5)