FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122850
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAAC252.6533453E-454.79315620
TGTATCC200.00754925251.389513
ATAGTAC200.0075736151.347688
ATCCAAT555.112972E-447.9381670
GTATCAA4800.044.252081
CCCTATA350.001390077539.1539152
TATCAAC5800.036.6224142
ATCAACG6100.034.821313
TCAACGC6200.034.812254
ACACCGT501.9294207E-434.2596746
GGTATCA2100.034.2596741
GTGGTAT807.4507625E-834.2596741
CAACGCA6450.033.4629365
AACGCAG6600.033.2215046
CAGAGTA6600.031.63846810
AGAGTAC6650.031.40058711
ACCGTGC553.3919828E-431.1198088
AGTACGG5800.030.69056713
GCAGAGT7150.030.6410399
TGGTATC902.0952211E-730.4530452
Produced by FastQC (version 0.11.5)