FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122850
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2380.19373219373219375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG250.001433279278.12018670
CTAAGCC200.006891606452.5871554
TCTAAGC200.00690297152.56525853
CCATAAT200.007226881651.95956840
TTGAGTA200.007549806551.3885843
TATAACA200.007549806551.38858416
GAAAGTT400.00922238448.82511570
GGTATCA2150.043.0580521
GTATCAA5100.041.00991
GTGGTAT702.2833774E-839.1851041
ATCAACG5650.036.987653
TGCTTAA450.001905473136.71333369
CAACGCA5950.036.2742925
TATCAAC5900.035.4348032
TCAACGC5950.035.1227264
AGAGTAC5900.034.83971811
AACGCAG6100.034.820686
ACGGGAT601.3983665E-534.25905616
CAGAGTA6100.034.25905610
CGCAGAG6300.033.715268
Produced by FastQC (version 0.11.5)