FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919012
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC116760.6084380921015606No Hit
GTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT99590.5189649673894692No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75650.39421327224634345No Hit
GATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA58940.30713721435822183No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49860.2598211996589912No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47730.24872173806104395No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46980.24481347693500613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46680.24325017248459105No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46260.24106154625400988No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC44880.2338703457821004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43550.22693969605192674No Hit
GTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG42570.22183290151390403No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG41540.21646555623414548No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41420.21584023445397946No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37150.1935892011097377No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC35550.18525157737419046No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT34730.1809785452097225No Hit
GTCTCCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTT34290.17868569868244702No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33960.1769660637869904No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33390.1739957853312017No Hit
GTCCCTGATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGT33010.1720155996940092No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT31900.1662313732274733No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT31370.16346953536507328No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT31150.16232311210143555No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31010.16159357002457517No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATG30560.15924861334895246No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29430.15336016658572224No Hit
CAGTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT28540.14872236338282407No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGAC28390.14794071115761653No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA27520.1434071282514127No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC27240.14194804409769193No Hit
ATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA26130.13616381763115604No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG26110.13605959733446168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25970.1353300552576013No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGC25910.13501739436751828No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT25330.1319950057633824No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC24960.1300669302745371No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24830.1293894983460239No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24120.12568967781337478No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA23880.12443903425304272No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23720.12360527187948799No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23700.12350105158279365No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23530.12261517906089175No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23520.12256306891254458No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23490.12240673846750307No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA23280.12131242535221248No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22160.11547608873732941No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21820.11370434369352564No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21760.1133916828034426No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21670.11292269146831807No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21320.11109883627616712No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTAC20880.10880598974889162No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT20850.10864965930385011No Hit
CACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTACCCTTTCACTTT20670.10771167663360104No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20320.10588782144145008No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT20090.10468928802946516No Hit
GCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTACCCTT20070.10458506773277083No Hit
TCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCGGGC19830.10333442417243874No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19690.10260488209557835No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19470.1014584588319406No Hit
CCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCG19250.10031203556830286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)