FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246939
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44060.35334527190183324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29000.23256951623134733No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28840.23128637407282956No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26770.2146857223970058No Hit
CCTCCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG26280.21075609953654506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25320.20305724658543842No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25130.20153351527219857No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25120.20145331888729118No Hit
GAGTATTAGGAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGTAAAGCCCCTAGCCTCCTGATCTATGAGGCGT24810.198967230955163No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24120.1934336803965551No Hit
GTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGAACTGGATCCG22650.181644811815173No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGCG22180.17787558172452703No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21350.17121928177721604No Hit
CATTATAATAGCTACCCTTGCGCGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT21240.17033712154323508No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21210.170096532388513No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGGAGCTGGTTGGCCTGGTATCAGCAGAAG21190.16993613961869827No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20840.16712926614694062No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20140.16151551920342536No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19380.1554205939504659No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA19000.15237313132398617No Hit
GTATTAGGAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGTAAAGCCCCT18850.15117018555037576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18740.1502880253163948No Hit
ATTATAATGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGC17920.14371192175399117No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17380.13938131696899367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17180.13777738927084646No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17120.1372962109614023No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16940.13585267603306977No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTGACAGACTTTAGATTCAGGGAGA16940.13585267603306977No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16560.13280521340659007No Hit
CACCTGCACTGTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGA16560.13280521340659007No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16450.13192305317260908No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16310.13080030378390603No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAACATTATAATAGCTAC15150.12149752313465215No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14980.120134184591227No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14970.12005398820631964No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14700.11788868581382088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14520.11644515088548837No Hit
GTGTAGGGTGCGTGGACTACGTAAAGAAATGCCCTTACTGGAGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCT14410.11556299065150742No Hit
GTTTATTACTGTGTAGGGTGCGTGGACTACGTAAAGAAATGCCCTTACTGGAGCCAGGGAACCCTGGTCACCGT14150.11347788464391602No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13980.11211454610049087No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13900.11147297502123199No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13690.10978885093817742No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13540.108585905164567No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13530.10850570877965963No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13410.1075433521607713No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13350.10706217385132713No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13320.10682158469660505No Hit
CCCTACACAGTAATAAACAGCCGTGTCTGCGGCGGTGACAGACTTTAGAT13120.10521765699845781No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTCCCACC13110.10513746061355045No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13100.10505726422864309No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13090.10497706784373576No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12970.10401471122484741No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12930.10369392568521797No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12750.10225039075688547No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12720.10200980160216337No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12640.10136823052290449No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12550.10064646305873823No Hit
ATTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAGTCTCACCATCAGCAGC12520.10040587390401615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGATA350.00521834456.44548870
GTATCAA28250.039.984241
CGACCGT451.0108491E-438.2736232
TATCAAC32200.034.8660932
CGCGACG400.002471945734.847351
CTTATAC2200.034.3329731
ATCAACG33400.033.614773
TACGGAT5900.033.4846170
TCAACGC33700.033.3155254
AACGCAG33900.033.2202576
CAACGCA34150.032.9770625
TATACAC3000.032.0453953
AATACGT553.3551455E-431.20553610
CGCAGAG36450.030.7958328
CGTATGC1251.200533E-1030.47334741
AGAGTAC37250.030.13323611
GCAGAGT38800.029.3729889
ACGCAGA38850.028.8933877
TGTTAGA7650.028.40720670
GAGTACG25000.028.01008812
Produced by FastQC (version 0.11.5)