FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246939
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44820.35944019715479264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27320.21909652356691067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26660.21380356216302482No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25560.2049819598232151No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25380.20353842489488258No Hit
GAGTATTAGGAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGTAAAGCCCCTAGCCTCCTGATCTATGAGGCGT24200.19407525147581398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23710.19014562861535328No Hit
GTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGAACTGGATCCG23420.1878199334530398No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGCG22810.18292795397369077No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21880.17546969017730618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21600.17322419139990006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21570.172983602245178No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21540.17274301309045592No Hit
CCTCCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG20910.16769064084129215No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20560.16488376736953453No Hit
CATTATAATAGCTACCCTTGCGCGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT19930.15983139512037076No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGGAGCTGGTTGGCCTGGTATCAGCAGAAG19170.15373646986741132No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19120.15333548794287452No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19090.15309489878815244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18940.151891953014542No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18260.14643859884084146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18000.14435349283325008No Hit
ATTATAATGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGC17960.14403270729362064No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17160.13761699650103174No Hit
GTATTAGGAGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGTAAAGCCCCT16900.13553189049344033No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTGACAGACTTTAGATTCAGGGAGA16430.13176266040279436No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15910.1275924483876116No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15860.12719146646307478No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15510.12438459299131714No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15420.12366282552715088No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15130.12133713036483743No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15080.12093614844030061No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14800.1186906496628945No Hit
CACCTGCACTGTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGA14650.11748770388928408No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14400.11548279426660006No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14360.1151620087269706No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14220.11403925933826756No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14210.1139590629533602No Hit
GTTTATTACTGTGTAGGGTGCGTGGACTACGTAAAGAAATGCCCTTACTGGAGCCAGGGAACCCTGGTCACCGT14040.11259572440993504No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13960.11195415333067615No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13890.11139277863632463No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13830.11091160032688045No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13800.1106710111721584No Hit
GAATTCAGTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAACATTATAATAGCTAC13670.1096284581683627No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.10730276300604921No Hit
GTGTAGGGTGCGTGGACTACGTAAAGAAATGCCCTTACTGGAGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCT13130.10529785338336517No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12780.10249097991160755No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12760.10233058714179283No Hit
CCCTACACAGTAATAAACAGCCGTGTCTGCGGCGGTGACAGACTTTAGAT12470.10000489197947934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA200.00755349751.4099817
TACGGAT6450.045.46436770
GTATCAA27600.039.9045751
TATCAAC31100.035.0588072
ATCAACG31150.034.8812683
TCAACGC31400.034.4888534
GTGGTAT4300.034.3104741
AACGCAG32250.033.5785036
CAACGCA32600.033.2185
TGTTAGA7100.031.6969970
TACACCG652.430678E-531.6331065
GCACTAG450.004749092730.49821
CTACGGA6950.030.3146369
CGCAGAG36050.030.039028
AGAGTAC37550.028.74779711
ACGCAGA37950.028.6253917
GCAGAGT38250.028.4008779
AGAGTCG500.00799269927.4153611
ACACCGC907.2764215E-626.653826
TTCGGGG12500.026.18747570
Produced by FastQC (version 0.11.5)