FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107445
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51450.46458289124967833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37910.34231948313460264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35800.3232666182067732No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35150.31739725223374526No Hit
GTCCCAACCAGGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGG34930.31541069759672036No Hit
CCAGGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT34770.313965930587975No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33400.30159511307559295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30990.279833310006366No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30190.2726094749626392No Hit
GTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCATCTGTCTT28200.2546401852913689No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28060.2533760141587167No Hit
GATCTACGATGTATCCAATTTGGCAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA27000.24380443272577873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26790.24190817602680043No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCGTCACTTGCCAGGCGAGTCAGGACATCAGTAA24540.22159113996631885No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24430.22059786264780643No Hit
GTGCAGCCACAGTTCGTTTTATTTCCACCTTGGTCCCTTGGCCGAACGAC24150.21806952038250205No Hit
GTTGGAGACAGAGTCACCGTCACTTGCCAGGCGAGTCAGGACATCAGTAACTATTTAAATTGGTATCAGCAGAAA24100.21761803069226915No Hit
TCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTAT23440.21165836678119454No Hit
CCCCTAAGGTCCTGATCTACGATGTATCCAATTTGGCAACAGGGGTCCCA23390.21120687709096164No Hit
ACATATTACTGTCAACAGTATGATCATCTCCGGTCGTTCGGCCAAGGGAC22240.2008226142156044No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCA22220.2006420183395112No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22130.19982933689709198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22070.19928754926881245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22040.1990166554546727No Hit
TGATCATACTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTC21790.19675920700350807No Hit
GAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCT21380.1930569915435981No Hit
GATTTTACTCTCACCATCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTATGATCATCTC20980.18944507402173472No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19830.17906081114637748No Hit
GGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCACTGAAC19630.17725485238544578No Hit
GCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19470.17581008537670043No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19060.17210786991679045No Hit
GTCCTGATCTACGATGTATCCAATTTGGCAACAGGGGTCCCATCAAGGTT18980.17138548641241777No Hit
CCTTAGGGGCATTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTACTGATGTCCTGACTCGCCTGGCAAGTGA18710.16894744208516No Hit
ATATTACTGTCAACAGTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGG18590.16786386682860097No Hit
AACCAGGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18500.16705118538618172No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTTATTTCCACCTTGGTCC18310.16533552456329662No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17940.16199450085557296No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17730.16009824415659468No Hit
TCGTAGATCAGGACCTTAGGGGCATTCCCTGGTTTCTGCTGATACCAATT17500.15802139158152323No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17410.15720871013910398No Hit
CACCATCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTATGATCATCTCCGGTCGTTCGG17360.15675722044887103No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCA17160.15495126168793935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17120.15459006993575303No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17090.15431917612161325No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16460.14863040602467842No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15490.1398715060341597No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15060.13598869469815658No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14960.1350857153176907No Hit
GCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCATCTGTC14960.1350857153176907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14820.13382154418503853No Hit
CAGTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCAT14590.13174469160996707No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCT14520.131112606043641No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14170.12795217821201052No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCGTCACTTGCCAGGCGAGTCAGG14080.12713949676959127No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13750.12415966481405397No Hit
GGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT13680.12352757924772788No Hit
AGTCAGGACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA13600.12280519574335519No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATC13270.11982536378781791No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC13270.11982536378781791No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13240.11955446997367815No Hit
TCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACC12930.11675523389423402No Hit
GCCTGAAGATGTTGCAACATATTACTGTCAACAGTATGATCATCTCCGGT12730.11494927513330233No Hit
ACTGTCAACAGTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCAC12540.11323361431041723No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12470.11260152874409113No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12380.11178884730167188No Hit
GATCAGGACCTTAGGGGCATTCCCTGGTTTCTGCTGATACCAATTTAAAT12300.11106646379729919No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12290.11097616585925262No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12270.11079556998315943No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12230.11043437823097309No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11880.10727395039934262No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTCTCACCATCAGTAGCCTGCAGCCTGAAGATGTTG11850.10700305658520287No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11730.10591948132864387No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11440.1033008411252929No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11400.10293964937310657No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11310.10212696793068732No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11310.10212696793068732No Hit
CAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11270.10176577617850095No Hit
GGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCATCTGTCTTCATC11180.1009530947360817No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11120.1004113071078022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22200.041.739431
TCGTATG503.7607952E-641.52987740
CCGAATT350.001384311839.2147311
GTGGTAT3350.038.929111
CTACGCT1950.037.01082628
TATCAAC25800.035.7823032
TACGCTG2050.035.21179629
ATCAACG26800.034.831313
TCAACGC26950.034.51014
CTTATAC2300.034.3190841
ACGAGAC2300.034.31908422
CAACGCA27550.033.883095
AACGCAG27950.033.275396
CGAGACT2300.032.831423
TATTAGC851.2654345E-732.3003122
CCCACGA2550.032.2959419
TATACAC2450.030.8171393
CGCAGAG30750.030.463158
CACGAGA2600.030.3578221
CTCGTAT802.7150545E-630.25326739
Produced by FastQC (version 0.11.5)