FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107445
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52020.4697298737183336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37040.3344635625245498No Hit
GTCCCAACCAGGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGG34880.31495920790648746No Hit
CCAGGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT32630.2946421718460059No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32060.2894951893773505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31730.2865153574218133No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29560.2669207048657044No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29410.26556623579500566No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29050.26231551002532855No Hit
GTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCATCTGTCTT27380.24723575437154893No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26490.23919923788540287No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCGTCACTTGCCAGGCGAGTCAGGACATCAGTAA25420.22953735851441834No Hit
GTTGGAGACAGAGTCACCGTCACTTGCCAGGCGAGTCAGGACATCAGTAACTATTTAAATTGGTATCAGCAGAAA25360.2289955708861388No Hit
GATCTACGATGTATCCAATTTGGCAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA25360.2289955708861388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24660.22267471522287788No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23770.21463819873673182No Hit
CCCCTAAGGTCCTGATCTACGATGTATCCAATTTGGCAACAGGGGTCCCA23750.2144576028606387No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23370.21102628121486847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21670.19567563174694905No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCA21410.19332788535773784No Hit
GTGCAGCCACAGTTCGTTTTATTTCCACCTTGGTCCCTTGGCCGAACGAC21370.19296669360555152No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21030.18989656371196764No Hit
GAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCT20420.18438838949112596No Hit
TCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTAT20320.18348541011066014No Hit
TGATCATACTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTC20050.18104736578340233No Hit
GATTTTACTCTCACCATCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTATGATCATCTC19950.1801443864029365No Hit
ACATATTACTGTCAACAGTATGATCATCTCCGGTCGTTCGGCCAAGGGAC19630.17725485238544578No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19460.17571978743865385No Hit
GCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19060.17210786991679045No Hit
GTCCTGATCTACGATGTATCCAATTTGGCAACAGGGGTCCCATCAAGGTT18460.16668999363399536No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18290.16515492868720344No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18110.16352956580236488No Hit
GGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCACTGAAC18040.1628974802360388No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17880.16145271322729346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17860.16127211735120028No Hit
AACCAGGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC17760.16036913797073443No Hit
ATATTACTGTCAACAGTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGG17750.16027884003268786No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17720.1600079462185481No Hit
CCTTAGGGGCATTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTACTGATGTCCTGACTCGCCTGGCAAGTGA17550.15847288127175616No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17230.15558334725426545No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTTATTTCCACCTTGGTCC17200.15531245344012568No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16910.15269381323677475No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16850.15215202560849522No Hit
CACCATCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTATGATCATCTCCGGTCGTTCGG16750.1512490462280294No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCA16420.1482692142724921No Hit
TCGTAGATCAGGACCTTAGGGGCATTCCCTGGTTTCTGCTGATACCAATT16350.147637128706166No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15890.1434834235560231No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCGTCACTTGCCAGGCGAGTCAGG15360.1386976328395541No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14520.131112606043641No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCT14420.13020962666317515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14340.12948724315880247No Hit
GGACACAGCGTGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT14320.1293066472827093No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATC13880.1253335380086596No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTCC13710.12379847306186764No Hit
AGTCAGGACATCAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA13320.12027685347805082No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13220.11937387409758499No Hit
GCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCATCTGTC13100.11829029884102597No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13010.11747761739860671No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12990.11729702152251355No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12950.11693582977032718No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12870.11621344626595452No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12710.11476867925720917No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12640.11413659369088308No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12610.11386569987674332No Hit
TCTTCAGGCTGCAGGCTACTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACC12590.11368510400065016No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12400.11196944317776504No Hit
CAGTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCACCAT12370.11169854936362528No Hit
GATCAGGACCTTAGGGGCATTCCCTGGTTTCTGCTGATACCAATTTAAAT11920.10763514215152896No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11900.1074545462754358No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTCTCACCATCAGTAGCCTGCAGCCTGAAGATGTTG11800.10655156689496996No Hit
ACTGTCAACAGTATGATCATCTCCGGTCGTTCGGCCAAGGGACCAAGGTGGAAATAAAACGAACTGTGGCTGCAC11730.10591948132864387No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11670.10537769370036434No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11410.10302994731115314No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11220.10131428648826803No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTCTCACCATCAGTAGCCTGCAGCCTGAAGATGTTGCAACATATTA11200.10113369061217486No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11170.10086279679803513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)