FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621523
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12320.19822275281847976No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11070.1781108663717996No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10790.17360580380774324No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10120.16282583267232267No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9780.15735539955882566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9610.15462018300207717No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9020.14512737259924413No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9000.14480558241609723No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8510.13692172292899862No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7740.12453280087784363No Hit
GTGTATTACTGTGTGAAACAGGACTACGGTGGCAGCTTGGGCGACAGGTT7740.12453280087784363No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7730.12437190578627018No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACTGCAGCCTCAGGGAGA7510.12083221377165447No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA7470.12018863340536069No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7420.1193841579474935No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7260.11680983648231842No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7100.11423551501714338No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCAGCATATCCGTTGGCACGGCGAAGAACCACTTCTCCCTGAGG6760.10876508190364635No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6640.10683434080476506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6460.10393822915644312No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC6420.10329464879014937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6240.10039853714182742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2450.040.4437181
GTATCAA19150.040.3235551
CGTACAA458.2830564E-539.5856863
TATCAAC22850.034.2427332
CGAACTA1253.6379788E-1232.89388329
TGTAAGG751.7983675E-631.8901042
TCAACGC24600.031.6678714
CAACGCA24850.031.486785
ATCAACG24800.031.4124873
AACGCAG25100.031.3092946
GTACGGA450.00484490830.3715271
GCCTAAC703.8794184E-529.53637136
ACGAGAC1302.2009772E-1028.91833922
GTATTAG1302.2009772E-1028.9113581
CTTATAC1302.2009772E-1028.9113581
CGCAGAG27300.028.6564318
CGGATTT605.640985E-428.55371929
AGCGAAC1452.5465852E-1128.33850327
AACGGAT2153.7232894E-828.1660770
GTGTAAG854.769565E-628.1383251
Produced by FastQC (version 0.11.5)