FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621523
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15510.24954828703040755No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12550.2019233399246689No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11630.18712099149991232No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11570.18615562095047167No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10420.1676526854195259No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9370.15075870080431458No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8600.1383697787531596No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7540.1213148990463748No Hit
GTGTATTACTGTGTGAAACAGGACTACGGTGGCAGCTTGGGCGACAGGTT7400.11906236776434662No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA7380.11874057758119973No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACTGCAGCCTCAGGGAGA7240.11648804629917156No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7060.11359193465084962No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6900.11101761318567455No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC6890.11085671809410111No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6720.1081215015373526No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6700.10779971135420571No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6680.10747792117105884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6660.10715613098791195No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6650.10699523589633851No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6580.10586897025532441No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6570.10570807516375098No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6340.10200748805756182No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG6270.10088122241654773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2500.050.6093031
TACCGTT306.51283E-445.704429
GTATCAA19850.040.311041
TCAACGC23850.033.369274
TATCAAC23900.033.3128622
ATCAACG24150.032.9573973
CAACGCA24300.032.7513245
AACGCAG24300.032.7513246
CGCAGAG25900.031.1237168
AGAGTAC25850.030.2589711
CAGAGTA26250.029.92800310
GCAGAGT27350.029.5985379
AACGGAT2552.1464075E-1028.9699970
ACGCAGA29050.027.5136977
ACTTACA500.00810264727.3365632
GACGGAC550.009294718526.57763167
TGTTAGA3305.456968E-1226.45606470
GTGTAGG1306.9194357E-926.304211
CCCTATA659.150912E-426.2851562
CCTATAC659.168126E-426.2766973
Produced by FastQC (version 0.11.5)