FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913029
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14990.16417879388277917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.040.3908581
GTATCAA40100.040.2323651
GTGGTAT4550.039.899141
TATCAAC45850.034.9894562
CAACGCA47950.033.723945
TCAACGC47750.033.7224
ATCAACG47800.033.6904143
AACGCAG49700.032.811636
CGCAGAG53350.030.438628
ACGCAGA53650.030.3321367
AGAGTAC52450.030.11357111
CAGAGTA54150.029.4838510
GCAGAGT56900.028.6597189
TGGTATC6450.025.9860382
AGTACGG39350.025.89031413
GAGTACG40400.025.38666512
TACGGGA10400.024.32563415
GTACGGG42250.023.62772614
CTCGCTA750.002092324722.80782123
GTACGGT750.002101005522.79158415
Produced by FastQC (version 0.11.5)