FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469799
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8410.1790127267192991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15000.040.149691
TCAACGC17500.034.5727164
ATCAACG17550.034.4778863
TATCAAC17600.034.3945852
CAACGCA17900.033.8001445
AACGCAG18250.033.155456
GGTATCA7300.031.4060061
CCGATAG450.00479381830.4358927
AATCGTA450.004823896630.39700118
AGAGTAC20350.029.39802611
ACGCAGA20600.029.3731547
CGCAGAG20900.028.7879628
CAGAGTA20800.028.76200910
ACGGGAC1103.988862E-827.97309916
GCAGAGT22100.027.5341879
GTACCGT500.008083343527.3485646
AAACCGT500.008083343527.3485646
AGTACGG14750.025.02822513
TTTCGTA851.2068324E-424.99861160
GAGTACG15900.023.65048612
Produced by FastQC (version 0.11.5)