FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613637
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTCCAGATATG58320.36141957577819545No Hit
GTGTATTACTGTGCCAGAACCTACGGTGACTACGGCATCCTGAATGATGC50330.31190410234767796No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48950.30335199304428445No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT45210.2801745373959571No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG42360.2625125725302531No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41820.2591660949767513No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39250.24323934069434452No Hit
GTATAATAATTGGCCTCCGGTCACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT38440.23821962436409178No Hit
GATCAGCACTGAGCACCGAGGACTCACCATGGAACTGGGGCTCCGCTGGG37550.23270413358146844No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC36820.2281801917035864No Hit
TTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC35830.22204498285549973No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34540.21405061981102316No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33010.20456893340943472No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32210.19961118888572835No Hit
GTCTCCAGCCATCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCT32040.19855766817444073No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31030.1922985157132614No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT31020.19223654390671507No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30400.18839429190084261No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29180.1808337315021904No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29170.18077175969564405No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28440.176247817817762No Hit
CTCCTGTGAAGCCTCTGGGTTCACCTTCAAGAACTACTTCATGAGTTGGGTCCGCCAGGCTCCAGGAAAGGGA28440.176247817817762No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAATTGG28200.17476049446065006No Hit
GCACTGAGCACCGAGGACTCACCATGGAACTGGGGCTCCGCTGGGTTTTC28110.1742027482017331No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27590.17098021426132395No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGGTTCACCTTC27260.16893514464529508No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26800.1660844415441639No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTGACCGGAGGCCAATTATTATACTG26710.16552669528524694No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGAAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT26180.16224218953829145No Hit
CTGGTATCCCAGCCAGGTTCTCCGGCAGTGGGTCTGGGACAGAGTTCACT25630.1588337401782433No Hit
GTGTGAGGTGCTGCTGGTGGAGTCTGGGGGAGGCCTGGTCGAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGC25600.1586478247586043No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA25420.15753233224077037No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC25390.1573464168211314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24300.15059148990758142No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23820.1476168431933576No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23270.14420839383330947No Hit
GTCCCAGACCCACTGCCGGAGAACCTGGCTGGGATACCAGTGGCCCTGGT23260.14414642202676314No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23210.1438365629940315No Hit
CTCCAGAGACAACGCCCAGAACTCAGCATATCTGGAGATGAACAGCCTGAGAGTCGAGGACACGGCTGTGTATTA23200.14377459118748517No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23060.14290698589583653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22560.13980839556852004No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC22540.1396844519554274No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT21540.1334872713007944No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21250.13169008891095085No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21060.1305126245865706No Hit
GTCTGGGGGAGGCCTGGTCGAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGGTTCACCTTCAAGAA20940.12976896290801462No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC20730.1284675549705417No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20610.12772389329198575No Hit
CCCTGAGACTCTCCTGTGAAGCCTCTGGGTTCACCTTCAAGAACTACTTC20410.12648445716105916No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG20100.12456333115812293No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA19920.12344783864028899No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT19510.12090699457188946No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTCCAGATA19120.11849009411658261No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC19110.11842812231003627No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18840.11675488353328536No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA18480.11452389849761749No Hit
GGCCAAAAGTGACCGGAGGCCAATTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTG18150.11247882888158862No Hit
GAGTATTAACAGAAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18070.11198305442921798No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17920.11105347733102304No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG17880.11080559010483772No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17810.1103717874590134No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC17630.10925629494117947No Hit
GTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCCAGAACTCAGCATA17520.10857460506916983No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG17490.10838868964953084No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG17360.10758305616442856No Hit
ATTCAGGATGCCGTAGTCACCGTAGGTTCTGGCACAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCAT17290.10714925351860424No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT17200.10659150725968727No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCGGAGAACCTGGCTGGGATACCA17160.10634362003350196No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC17040.105599958354946No Hit
TCTATGATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCTCCGGCAGTGGGTCTGGGACAGAGTTCACT16980.10522812751566801No Hit
GTATTACTGTGCCAGAACCTACGGTGACTACGGCATCCTGAATGATGCCT16840.10436052222401941No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC16790.10405066319128775No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16670.10330700151273178No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG16580.10274925525381483No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16400.10163376273598088No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.10150981912288824No Hit
GGACTGGAGTGGGTCTCATTTATTAGTGCTAGCGGGAAGTTCTTCTACTA16280.10089010105742494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)