FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613637
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50330.31190410234767796No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50310.3117801587345853No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTCCAGATATG49300.30552100627340595No Hit
GTGTATTACTGTGCCAGAACCTACGGTGACTACGGCATCCTGAATGATGC47170.2923210114790377No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT44670.2768280598424553No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG39050.2419999045634179No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT38910.24113229927176927No Hit
GATCAGCACTGAGCACCGAGGACTCACCATGGAACTGGGGCTCCGCTGGG38610.2392731450753794No Hit
TTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC35250.2184506180758126No Hit
GTATAATAATTGGCCTCCGGTCACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT35140.21776892820380297No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31950.19799992191552374No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA31850.19738020385006044No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31470.19502527520129992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29850.1849858425407945No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT29660.18380837821641421No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29630.18362246279677522No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29320.181701336793839No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCGGTTTATTACTGTCAGCAGTATAATAATTGG28380.175875986978484No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28340.1756280997522987No Hit
CTCCTGTGAAGCCTCTGGGTTCACCTTCAAGAACTACTTCATGAGTTGGGTCCGCCAGGCTCCAGGAAAGGGA28210.1748224662671964No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTGACCGGAGGCCAATTATTATACTG27570.1708562706482313No Hit
GTCCCAGACCCACTGCCGGAGAACCTGGCTGGGATACCAGTGGCCCTGGT27560.17079429884168498No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27560.17079429884168498No Hit
GTGTGAGGTGCTGCTGGTGGAGTCTGGGGGAGGCCTGGTCGAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGC27310.16924500367802672No Hit
GTCTCCAGCCATCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCT26910.16676613141617352No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGGTTCACCTTC26750.16577458251143226No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26630.1650309208328763No Hit
GCACTGAGCACCGAGGACTCACCATGGAACTGGGGCTCCGCTGGGTTTTC25600.1586478247586043No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACAGAAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT25450.15771824766040937No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT25350.15709852959494608No Hit
CTGGTATCCCAGCCAGGTTCTCCGGCAGTGGGTCTGGGACAGAGTTCACT25340.15703655778839973No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA24950.15461965733309288No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24870.15412388288072223No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24610.15251261591051765No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT24350.1509013489403131No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23970.14854642029155257No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23600.14625346344933834No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT23460.14538585815768976No Hit
CTCCAGAGACAACGCCCAGAACTCAGCATATCTGGAGATGAACAGCCTGAGAGTCGAGGACACGGCTGTGTATTA22280.1380731849852228No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21850.13540839730373064No Hit
GTCTGGGGGAGGCCTGGTCGAGCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGCCTCTGGGTTCACCTTCAAGAA21790.13503656646445267No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21680.13435487659244305No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21570.1336731867204334No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT21560.13361121491388708No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21500.13323938407460909No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21360.13237177878296047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21260.13175206071749718No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT20630.1278478369050784No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA20520.12716614703306878No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20500.1270422034199761No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20490.12698023161342978No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20180.12505910561049358No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20080.12443938754503027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19500.12084502276534313No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA19420.1203492483129725No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT19290.11954361482787021No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18930.11731262979220235No Hit
CCCTGAGACTCTCCTGTGAAGCCTCTGGGTTCACCTTCAAGAACTACTTC18790.11644502450055372No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCGGAGAACCTGGCTGGGATACCA18760.11625910908091472No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC18600.11526756017617346No Hit
GGCCAAAAGTGACCGGAGGCCAATTATTATACTGCTGACAGTAATAAACCGCAAAATCCTCAGACTGCAGGCTG18440.11427601127143217No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17950.11123939275066201No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17700.10969009758700377No Hit
GGACTGGAGTGGGTCTCATTTATTAGTGCTAGCGGGAAGTTCTTCTACTA16830.10429855041747307No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16740.1037408041585561No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTCTCTTGTCC16670.10330700151273178No Hit
CAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATCTCCAGATA16580.10274925525381483No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16340.10126193189670292No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16260.10076615744433227No Hit
ATTCAGGATGCCGTAGTCACCGTAGGTTCTGGCACAGTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCAT16190.10033235479850797No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16150.10008446757232264No Hit
CTGCTACTCTGGCTCCCAGATACCGCTGGAGAAGTAGTGATGACGCAGTC16140.10002249576577633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)