FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423635
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5960.140687148134597No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC5480.12935663955999857No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT4980.11755402646145857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACA200.00749082951.51249329
TACCGTG200.007585987451.3483057
TTCTAAT451.9546824E-645.66452
GTATCAA12300.038.4522551
CCTATAC400.002692423534.2402883
TCTAATG501.9438572E-434.2402843
CGCCTAA450.002984470233.5276968
GGTATCA5250.033.293441
AACGCAG14600.032.82546
TCAACGC14600.032.5909354
ATCAACG14550.032.2399983
CAACGCA14900.031.9347385
ACGCAGA15900.030.1415637
AGAGTAC16500.029.45696311
CAGAGTA16450.029.33842810
CGCAGAG16550.028.9543388
TATCAAC16250.028.8740162
TGATAAC605.556522E-428.62483230
GCAGAGT17350.027.816559
GTGGTAT2100.027.7445371
Produced by FastQC (version 0.11.5)