FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304303
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACGGCACTCAGGCTGCTGTCATAGGA77670.337065047435168No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACGGCACTCAGG68810.29861524287387553No Hit
CTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGT64350.2792601493813965No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG54710.23742537331245067No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53380.2316535629211957No Hit
GTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGT49450.2145985141710964No Hit
GAGCAGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT47850.20765498287334608No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46600.20223034904697865No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45200.19615475916144706No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41070.17823176899912904No Hit
GTCCTATGACAGCAGCCTGAGTGCCGTGGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG38000.16490886832157056No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37210.16148049974330633No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36920.16022198469558907No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36780.15961442570703593No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35740.1551011303634982No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC34490.14967649653713075No Hit
ATTATAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG34380.14919912876041042No Hit
GGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGTCCCCAAACTCCTCATCTATTATAACACCAATCGG34070.14785381957147128No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33410.14498961291114928No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32490.14099708241494283No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31260.13565924272979726No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT30490.1323176682927549No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30190.1310157561744267No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCACGGCACTCAGGC30140.130798770821372No Hit
GATGTACACTGGTACCAGCAGTTTCCAGGAACAGTCCCCAAACTCCTCAT29630.12858552022021408No Hit
GTGATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATGCA29210.12676284325455464No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC29200.12671944618394368No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29140.12645906376027805No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28820.12507035750072798No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28520.12376844538239978No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26580.11534941368387751No Hit
GCACTGAACACAGAGGACTCTCCATGGAATTGGGACTGAGCTGGATTTTC26240.11387391328310556No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26200.1137003250006618No Hit
GGACAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG26060.11309276601210866No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26050.11304936894149771No Hit
GTCCCCAAACTCCTCATCTATTATAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT25900.11239841288233361No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25870.1122682216705008No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25870.1122682216705008No Hit
CCCCAGGACAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATC25750.11174745682316953No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25750.11174745682316953No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA23530.10211330714754091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.044.2982141
GTATCAA38900.044.1796461
TATCAAC44000.039.057132
TCGTATG1200.037.48478340
ATCAACG49250.034.823753
TCAACGC49150.034.7544674
CAACGCA49450.034.474725
AACGCAG51250.033.3983046
CGCAGAG55350.030.8594538
AGAGTAC55900.030.6174311
CAGAGTA56750.030.15884610
CGTATGC1501.8189894E-1230.00483541
ACGCAGA57400.029.8773217
GCAGAGT58700.029.2743079
TGGTATC5450.028.4359952
TTCGGGG20250.026.17577770
AGTACGG46700.025.8086913
TGCAAGG29350.025.14676570
GAGTACG47900.025.0902312
CGGCTTT19000.024.65011216
Produced by FastQC (version 0.11.5)