FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119249
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCTGCGGCGGTCAGAGAGGTCAACTTCAGGGAGAACTGGTTCCTGGACGTGTCTACGGA36810.3288812409034987No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26090.2331027322785189No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25200.22515097176767634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23420.2092474507459913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21800.1947734597037835No Hit
ATATATTACTGTGCGAGACACGTAGAGATGCTTCCAATCAGGTTCGACCC20490.18306918299681305No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19910.1778871368212078No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19790.1768149893365998No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19110.17073948692382124No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17960.16046474019632806No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17640.15760568023737345No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16290.14554402103553365No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16090.143757108561187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15940.14241692420542704No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACACATTTCA15850.14161281359197103No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15850.14161281359197103No Hit
CACTTGCACCGTCTCTGGTGGCTCCTTCGGCAGTAGTGAATACTACTGGG15570.13911113612788575No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14910.13321432496254185No Hit
GTACTGTAACTCTGTTGACAGTAATAAGTAGCAAAATCTTCAGGTTGCAG14860.13276759684395517No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14570.13017657375615255No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG14540.12990853688500056No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14460.1291937718952619No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14070.12570929257028596No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13870.12392238009593932No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13860.12383303447222199No Hit
GTCTCTGGTGGCTCCTTCGGCAGTAGTGAATACTACTGGGGCTGGATCCG13700.12240350449274467No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13620.12168873950300603No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13550.1210633201369847No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13160.11757884081200877No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCTACTTATTACTGTCAACAGAGTTACAGTACCTTGCTCTCTTT13160.11757884081200877No Hit
GTTGATGGTGAGAGTGAAATGTGTCCCAGATCCACTGCCACTGAACCTTG13150.11748949518829144No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12970.11588127396137947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12660.11311155962614218No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12600.11257548588383817No Hit
GAGTTACAGTACCTTGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12580.11239679463640351No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12570.11230744901268618No Hit
CCATAGATCAGGAGCTTAGGGGCTTCCCCTACTTTCTGCTGATACCAATT12440.11114595590436087No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT12340.11025249966718756No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12330.11016315404347023No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11940.10667867471849428No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11840.10578521848132097No Hit
CAGTAATATATAGCCGTGTCTGCGGCGGTCAGAGAGGTCAACTTCAGGGA11820.10560652723388629No Hit
GTTACAGTACCTTGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA11740.10489176224414763No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11720.10471307099671297No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA11680.10435568850184365No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG11650.10408765163069165No Hit
GTCCGTAGACACGTCCAGGAACCAGTTCTCCCTGAAGTTGACCTCTCTGACCGCCGCAGACACGGCTATATATTA11620.10381961475953966No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11500.10274746727493167No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11280.10078186355315037No Hit
GTCTACGGACATGGTGACTCGACTCTTCAGAGACAGGTTGTGGTAGGTATTCCCATTGTAAGAGATACTCCCAAT11280.10078186355315037No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11200.10006709856341173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG200.007171565752.0887247
GTATCAA19300.043.47591
TATCAAC22150.037.230952
ATCAACG23450.034.859893
TCAACGC23450.034.8567734
CAACGCA23600.034.6352275
AACGCAG24100.034.0591626
GTGGTAT2500.031.6376291
AGAGTAC26100.031.58085311
CGCAGAG26250.031.4003898
GCAGAGT26800.030.7559769
CAGAGTA29000.028.54119510
ACGCAGA28900.028.5211127
AGTACGG22050.028.5033313
GAGTACG22700.027.68715512
CCGTTAA500.00790586127.47656616
TCCGACT500.00790758527.47534213
TTCGGGG11900.026.47609970
TACGGGG12500.026.10157215
GTTACTA1300.001024080725.7827870
Produced by FastQC (version 0.11.5)