FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291693
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATACTGTTGGCAGTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC34040.26353011125708664No Hit
GTATAGTAGTTATCCGACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33000.25547866249952583No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCGGTTCATTTGCAGAAAGA29610.22923403626093816No Hit
GTCTATTACTGTGCGAGAGATTGCAGTCATGGTAGCTGCTACTCCAACTATTACTACGGTATGGACGTCTGGGGC25730.1991959389731151No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23880.1848736503178387No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17760.1374939710906539No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17480.1353262733482337No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17200.13315857560581346No Hit
GTGTAGCATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAG16990.1315328022989983No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16220.12557163350734268No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15100.11690084253766181No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15030.11635891810205674No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14740.11411380258312152No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14650.11341704259448646No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14440.1117912692876713No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14180.10977840709828109No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13870.10737845602631585No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13460.10420432718920053No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13420.1038946560831405No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13390.10366240275359548No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13340.10327531387102043No Hit
GTACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG13270.10273338943541538No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12930.10010118503390511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)