FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947736
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC117390.3982378340529817No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCCCGTTTTCGG96890.3286929358667126No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGAGCAGAGGGTCAC95730.32475771235958717No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGGGACCATTCAGGCTGTCATCCCATG94960.3221455381350298No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG92760.3146821832077228No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGAGCAGAGG87890.2981610293459116No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG86590.29375086507068476No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT84570.28689814827379384No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC80940.27458361264373743No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGGGACCATTCAGG80610.2734641094046414No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG68950.23390832828991467No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC68120.23109260802188528No Hit
CCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT63400.21508031926875407No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG62040.21046660895005523No Hit
CCTCACTCACTGTGCAGGTTCCTGGGCCCAGTCTGTACTGACTCAGCCAC62010.21036483592831923No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT55860.1895013664724385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC54520.184955504834897No Hit
GCATGGGATGACAGCCTGAATGGTCCCGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG53470.18139344907413688No Hit
GTCTGGGGGCCGTTCCTGGGATCTGCTGGTACCAGTTTACAGTATTAGTT52700.17878127484957948No Hit
CTCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGATCTGCTGGTACCAGTTTA52060.17661011705254473No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA51720.17545668947287005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50160.17016449234259784No Hit
GAACTAATACTGTAAACTGGTACCAGCAGATCCCAGGAACGGCCCCCAGA49750.16877359437887246No Hit
GTGCAGGTTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT49450.16775586416151242No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC48660.16507584125579766No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG46350.1572393185821254No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT46100.15639121006765871No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT45770.15527170682856267No Hit
GTATTAGTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT45360.1538808088648373No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGGGACCATTCAGGC45070.15289700298805592No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA43800.1485886117345651No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG42340.1436356580100796No Hit
GTAATAATCAGCTTCATCGTCAGACTGGAGCCCACTGATGGCCAGGGAGG41740.14160019757535955No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG41680.14139665153188752No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC41660.14132880285073018No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41230.13987005620584747No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41000.1390897963725381No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGACGATG40050.13586698401756467No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA40030.13579913533640733No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39950.13552774061177797No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39330.13342443149590058No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT39080.13257632298143388No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG38720.13135504672060183No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTCCCGTTTTCGGCGGAGGGACC38340.13006592177861245No Hit
GGCCATCAGTGGGCTCCAGTCTGACGATGAAGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCC37830.1283357804091004No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37440.12701273112653236No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA37170.12609677393090835No Hit
GTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGAGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG36000.12212762608320421No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35820.12151698795278819No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC34990.11870126768475874No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC34840.11819240257607871No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC34790.11802278087318539No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC34050.11551237967036398No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC34000.11534275796747064No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC33310.11300197846754255No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33230.1127305837429132No Hit
CTGTGCAGGTTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGAGCAGAGGGTCAC33020.11201817259076119No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC32900.11161108050381716No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC32740.11106829105455848No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT32510.11028803122124912No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32390.10988093913430512No Hit
GCCCCCAGACTCCTCATCTATACTAATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGT32190.10920245232273174No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA32180.10916852798215307No Hit
GCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG32070.10879536023578773No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC32040.10869358721405174No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAACTAATACTGTAAACTGGTACCAGCAGATCCCAGGAACGGCCCC31660.10740446227206235No Hit
GTATAGCAGTCACCTCCACTACAATATTTAAGTCGGCCTTTTGCACAGTA31440.10665812677933166No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC31090.10547077485907828No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC30880.10475836370692627No Hit
CCCTCAGCGTCTGGGACCCCCGAGCAGAGGGTCACCATCTCTTGTTCTGG30080.10204441646063285No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGTTCCTGGGC29870.10133200530848081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)