FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063755
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC97240.4711799607996104No Hit
GTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT88700.4297990798326352No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70310.34068966519766153No Hit
GATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA50630.24532950858992467No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT48610.23554152503567527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43810.2122829502533004No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41580.20147740405232212No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT41510.2011382165034125No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC41190.19958764485125416No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40750.19745560882953647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40190.1947421084382594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39610.19193169731872242No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39590.19183478659046252No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG39240.19013884884591437No Hit
GTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG38370.18592323216660894No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37810.18320973177533187No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36420.17647443616126912No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT35630.17264646239500328No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC34110.16528124704725125No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34010.16479669340595177No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32810.15898204971035806No Hit
CTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGCTATGGCATGAACTGGGTCCGCCAGGCTCCAGGCAAGGGG31880.15447570084627293No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT31470.15248903091694507No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG31100.15069618244413702No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT30180.14623828894418184No Hit
GTCTCCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTT30130.1459960121235321No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28860.13984218087902875No Hit
GTCCCTGATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGT28480.13800087704209074No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT27880.1350935551942939No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT27690.13417290327582487No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27670.134075992547565No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA27270.1321377779823671No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC27110.13136249215628795No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATG26220.1270499647487226No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC26120.12656541110742311No Hit
ATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA25870.12535402700417442No Hit
CTTTTATACGGTCTGATGAAAGTAAAGAATACTATGCCGACTCCGTGAAG25190.122059062243338No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA24850.12041157986291977No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT24710.1197332047651005No Hit
CAGTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT24450.11847336529772186No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC24290.1176980794716427No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23710.11488766835210576No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGAC23690.11479075762384586No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGC23120.11202880186843883No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22960.11125351604235967No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA22690.1099452212108511No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22480.10892765856412219No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG22440.1087338371076024No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21690.10509968479785632No Hit
GTATATTTCTGTGCGAAACGAACGGAGAGCTCCTCTCATCATCGGGCCTT21340.10340374705330817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21230.10287073804787873No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC21100.10224081831418942No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA20950.10151398785224021No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20880.10117480030333058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT200.007303702651.85033439
GTATCAA36100.043.8929981
GGTATCA12850.043.599951
TATCAAC40300.039.1479572
ATCAACG42400.037.1288643
TCGTATG1500.036.90192440
CAACGCA42900.036.616895
TCAACGC43000.036.6107834
ACCGTAC1700.036.4280327
AACGCAG44000.035.7795876
CGTACTA1800.034.41927329
GTGGTAT4250.033.9673651
TGGTATC4500.033.607922
CGTATGC1700.032.58038341
CGCAGAG48850.032.2945028
AGAGTAC49100.031.91929411
GTACTAG1950.031.77549630
CAGAGTA49600.031.6668210
GCAGAGT50500.031.1032149
CCTTTGT17200.030.17631370
Produced by FastQC (version 0.11.5)