FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3827387
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT133120.3478090927308892No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGCGCCTCTTG88740.23185531016330463No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG87000.22730912761108296No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC80880.2113191062205102No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC78450.2049701271389593No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT78270.20449983239217775No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT67410.17612538266969083No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG66410.17351263407646No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGACTTCACACTGCA65940.1722846422376415No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65850.17204949486425072No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG65550.17126567028628148No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC62350.1629048747879428No Hit
GTGATGTAGGAACCACCTATTTGGACTGGTATGTTCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACACA62290.16274810987234894No Hit
GGTCTAGTCAGCGCCTCTTGGATGGTGATGTAGGAACCACCTATTTGGACTGGTATGTTCAGAAGCCAGGGCAGT61480.16063178351183197No Hit
GCTTACAACGTGTGCAGTTTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCAC60250.15741810274215803No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58120.15185294823857634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT57330.14978887684992398No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT53320.13931175499106832No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51840.1354448870730867No Hit
CGTTGTAAGCAGTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTTGCAGTGTGAAGTCAGTGCCTGAC50450.1318131665284958No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC50350.13155189166917272No Hit
GTCTGGGACTCCAGAGGCCCGGTAGGAAAGTGTGTAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGAACATA49370.1289913980478065No Hit
GGTATGTTCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTACACACTT48130.12575158979220027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG47270.12350462600202175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC46800.12227663416320325No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG46790.12225050667727094No Hit
GACTTCACACTGCAAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCTTACAACGTGTGCAGTTT45620.11919359082319086No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC45010.11759981418132004No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT44970.11749530423759082No Hit
CTTTCCTACCGGGCCTCTGGAGTCCCAGACAGGTTCAGTGGCAGTGGGTC43900.11469966324283382No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA43380.11334103397435379No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG42140.11010122571874754No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACGGAAACTG42110.11002284326095062No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA41490.10840293913314751No Hit
GTCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG41010.1071488198083967No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG40980.10707043735059976No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG40570.10599921042737513No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCACTGAGGGAATTGTGATGA40470.10573793556805204No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG39190.10239361736871656No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39020.10194945010786732No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG38840.10147915536108579No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT38810.10140077290328885No Hit
GCTAATGCTCTGGGTCCCTGGATCCACTGAGGGAATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCC38560.10074758575498113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.044.595651
GTATCAA75450.039.5195271
TATCAAC83900.035.497862
TGGTATC11650.033.06272
ATCAACG90250.032.9621163
TCAACGC90650.032.81714
CAACGCA92750.032.223655
GTGGTAT13000.032.010611
AACGCAG95350.031.381466
ACCGATC5000.029.59607727
CGCAGAG106800.028.0781658
AGAGTAC105900.028.023811
ACGCAGA109800.027.2483637
GCAGAGT111350.026.8378319
TCGTATG1607.6397555E-1125.9411140
CAGAGTA118800.025.09692410
CCGATCA6300.024.03897728
AGTACTT49900.023.01629313
GAGTACT50100.022.9933512
CGATCAG6450.022.95109729
Produced by FastQC (version 0.11.5)