FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148943
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC650.057.821461
CTCGTAT653.6379788E-1253.2081839
AATCGAG200.007633506351.2508435
GTGGTAT750.050.1119351
TCTCGTA707.2759576E-1249.3572638
TCGTATG652.055458E-1047.91993740
GGTATCA2750.043.485561
TCCGAGC950.043.15860413
AATCTCG901.8189894E-1242.11348336
TTATACA903.6379788E-1241.7599452
TATACAC903.6379788E-1241.7599453
GTATCAA6950.040.8040241
CGTATGC702.057459E-839.66088541
CGAGACA1050.039.074523
GCTAGTC350.00140730339.0613720
AGGCAAT901.2732926E-1038.13003532
ACGAGAC1001.0913936E-1137.59657322
ATCTCCG1001.0913936E-1137.58394610
ACACATC1001.0913936E-1137.5839466
CACGAGA1100.037.2858621
Produced by FastQC (version 0.11.5)