FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148943
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4530.3041431957191677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCA150.002075491571.205463
CATAAGT150.002450609868.2853825
TAATCTT350.005398161255.917770
TTAGGAC200.00768578151.162443
TGGTTAC200.00768578151.1624415
TAACCGT200.00768578151.162447
GTGGTAT1150.050.5057561
GGGTATC306.6030136E-445.554211
GTACCCT405.2755415E-542.707071
GTATCAA7400.038.782641
TGGTATC1550.037.4216652
TATCAAC8000.036.2522352
ACTGAGC400.00273821134.108293
ATCAACG8650.033.9111373
AACGCAG8650.033.9111376
CAACGCA8550.033.908835
TCAACGC8600.033.7116854
GGTATCA3400.033.1607861
ACGCAGA9000.032.592377
ACGGGCC851.3226054E-732.10192516
Produced by FastQC (version 0.11.5)