FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988830
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36040.36447114266355185No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25960.26253248788972827No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATT25480.2576782662338319No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23660.2392726757885582No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT20950.21186654935630997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20660.20893379043920593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20270.20498973534379014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19800.20023664330572497No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19170.19386547738236098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18210.18415703407056824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17270.1746508499944379No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGAT16790.1697966283385415No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16580.16767290636408685No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT16520.1670661286570998No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAA16350.16534692515396984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15470.15644751878482652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15430.15604300031350182No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTCCGCCGAAACCG15390.15563848184217713No Hit
GACTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGAAGTATGATAACTGG14070.1422893722884621No Hit
GAGTATTAGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC14050.14208711305279978No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13940.14097468725665685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13880.14036790954966982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13870.14026677993183864No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTA13730.1388509652822022No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13360.13510916942244877No Hit
GTTATCATACTTCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGTTGATGGTGAGAGTGAAGT13250.13399674362630584No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12870.1301538181487212No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGGTTTAGGGAGA12690.1283334850277601No Hit
GTATGATAACTGGCCCGGTTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT12380.12519846687499367No Hit
CACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGAAGTATGATAACTGGCCCGGTTTCGG11880.12014198598343498No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC11530.11660244935934388No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11450.11579341241669447No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11000.11124257961429164No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10850.10972563534682402No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTTCTACTGGAGCTGGATCCG10780.1090177280220058No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10720.10841095031501875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10540.10659061719405763No Hit
GTTTATTACTGTCAGAAGTATGATAACTGGCCCGGTTTCGGCGGAGGGAC10500.10618609872273294No Hit
TCTATGGTGCATCCATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT10420.10537706178008353No Hit
CATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAG10360.10477028407309648No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10250.10365785827695358No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10050.10163526592033008No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9890.10001719203503129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG651.0981239E-842.63326340
GTATCAA22200.041.0894471
CAGTCGA350.001258742539.9801354
TCGCGTA459.9214194E-538.39422238
TATCAAC25000.036.6245962
GGTATCA9400.035.0223271
CGCGTAT501.8329258E-434.5900539
ATCAACG26600.034.421613
TCAACGC26750.034.10044
CAACGCA26950.033.8473325
AACGCAG27700.032.9308856
ACGGGTA751.7493239E-632.00651616
CACGAGA2650.031.06224421
TCGAACG450.004389943630.99174149
TGTTAGA4750.030.6663770
ACCGATC2600.030.38039427
TATCTCG1251.2551027E-1030.3633636
ACGAGAC2850.030.08587322
CGATCAG2650.029.82530629
CGCAGAG30600.029.808488
Produced by FastQC (version 0.11.5)