FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988830
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36580.36993214202643526No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATT24680.24958789680733798No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24290.24564384171192216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23820.24089074967385696No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21850.22096821496111566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20660.20893379043920593No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19060.19275305158621805No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT18840.19052819999393222No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17930.18132540477129536No Hit
CATCTATGGTGCATCCATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16940.1713135726060091No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16550.1673695175105933No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGAT16350.16534692515396984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15970.1615039996763852No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT15940.1612006108228917No Hit
GACTTCACTCTCACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGAAGTATGATAACTGG15890.16069496273373585No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAA15410.15584074107783946No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTCCGCCGAAACCG14610.14775037165134552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13970.1412780761101504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13590.13743515063256576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13560.13713176177907224No Hit
GAGTATTAGCAACAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC13340.1349069101867864No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAACAACTTAGCCTGGTA12880.1302549477665524No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12820.12964817005956536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12660.12803009617426656No Hit
GTTATCATACTTCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGTTGATGGTGAGAGTGAAGT12640.1278278369386042No Hit
GTATGATAACTGGCCCGGTTTCGGCGGAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT12380.12519846687499367No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12100.1223668375757208No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12020.12155780063307141No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11950.12084989330825319No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11780.11913068980512322No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGGTTTAGGGAGA11500.11629906050585036No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11480.116096801270188No Hit
CACCATCAACAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGAAGTATGATAACTGGCCCGGTTTCGG11470.11599567165235683No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11130.1125572646460969No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10870.10992789458248638No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT10510.1062872283405641No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10490.10608496910490176No Hit
GTTTATTACTGTCAGAAGTATGATAACTGGCCCGGTTTCGGCGGAGGGAC10470.10588270986923941No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTTCTACTGGAGCTGGATCCG10310.10426463598394063No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10310.10426463598394063No Hit
CATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAG10230.10345559904129122No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10140.10254543248081065No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10020.10133187706683658No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC9890.10001719203503129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23600.042.432371
GGTATCA9200.037.27681
TATCAAC26800.036.951992
ATCAACG27300.036.138723
TCAACGC27250.036.0756724
CAACGCA28350.034.675915
AACGCAG28650.034.5519266
AGAGTAC30350.032.8422811
CGCAGAG30900.032.0360078
ACGCAGA31000.031.9326697
GCAGAGT31700.031.3355839
CGTACAT553.3968125E-431.13912212
AGTACGG25350.028.24017513
GAGTACG25600.027.9643912
TGTTAGA5300.025.90111570
GACAGTA10300.025.32274270
GAGTACT11650.024.10943212
TACGGGG14150.023.9662315
CAATCGT750.001694134223.6509965
GTACGGG30900.023.05705814
Produced by FastQC (version 0.11.5)