FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765974
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24650.3218124897189722No Hit
GTATAATGATATTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24510.3199847514406494No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23190.3027517905307491No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23120.3018379213915877No Hit
TCATTATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTC21610.28212445853253504No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20990.2740301890142485No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19400.2532723042818686No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19280.25170567147187767No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16450.21475924770292462No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16150.2108426656779473No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16010.20901492739962452No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15700.2049677926404813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14750.19256528289471966No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14190.1852543297814286No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13980.18251272236394445No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13920.181729405958949No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13540.17676840206064434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13390.1748101110481557No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.17467955831398976No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13140.1715462926940079No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13110.17115463449151014No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12600.16449644504904865No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12410.16201594309989634No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12050.15731604466992352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12000.15666328099909396No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11960.15614107006243033No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAATCACCATCACTTGCCAGGCGAGTCACGACATTACCAA11770.153660568113278No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11360.14830790601247562No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11130.14530519312665965No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10920.14256358570917552No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10860.14178026930418003No Hit
GTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA10720.13995253102585728No Hit
GTCTGCATCTGTCGGAGACAGAATCACCATCACTTGCCAGGCGAGTCACG10490.1369498181400413No Hit
ATGATATTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA10060.1313360505709071No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10030.13094439236840938No Hit
GTCTCTACTGGCTCCATCAACAGTGGTGATTACTACTGGAGTTGGATCCG9760.12741946854592975No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA9720.12689725760926612No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9710.1267667048751002No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC9640.12585283573593883No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9620.125591730267607No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9560.12480841386261152No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9380.12245846464762511No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9120.1190640935593114No Hit
GGCTGTAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCC9080.11854188262264775No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9050.11815022442015002No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9040.11801967168598411No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9030.1178891189518182No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT8980.11723635528098865No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8730.11397253692684087No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8720.11384198419267495No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA8620.11253645685101582No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8530.11136148224352262No Hit
GCCTACAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATAATGATATTCCCCTCACTTTCGGCGGAGGGA8500.11096982404102489No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8490.11083927130685897No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA8290.10822821662354075No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8100.10574771467438843No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGCCTTCTGCTGATACCAATT8090.10561716194022251No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT8020.10470329280106114No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7840.10235334358607473No Hit
ATATTACTGTCAACAGTATAATGATATTCCCCTCACTTTCGGCGGAGGGA7800.10183113264941109No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7790.10170057991524517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC7770.10143947444691333No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7730.10091726351024968No Hit
GCAGAAGGCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTT7720.10078671077608378No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7670.10013394710525422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT150.002301544669.4024345
TATAAGC200.0075211751.4638753
GCCTACG200.00752311251.4605181
TCGTATG503.7785576E-641.49895540
GTATCAA19600.041.1334081
GTGGTAT3100.040.947081
AGTCCGA350.001385654839.20545210
CGTATGC557.2349703E-637.7536541
CGTACTA557.595547E-637.48701529
GACCGTA557.633114E-637.46006826
ACGAGAC655.6898534E-736.94842522
GACAGGT1151.8189894E-1235.7986221
TATCAAC22650.035.291542
ATCTCGT501.8663524E-434.48261337
GTACTAG601.374069E-534.38108430
ACCGTAC601.383951E-534.3451327
ATCAACG23650.033.9465753
TCAACGC24050.033.3819734
CAACGCA24150.033.245925
TACTAGA652.3706198E-531.74677331
Produced by FastQC (version 0.11.5)