FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419451
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC200.00755148151.4074633
GGTATCA7450.042.776951
GTATCAA18800.041.0117071
TATCAAC21100.036.2207452
GTGGTAT1750.035.246631
ATCAACG22650.033.893373
CAACGCA22600.033.2101335
AACGCAG22950.032.703666
TCAACGC23400.032.5141224
CGCAGAG24900.029.997748
AGAGTAC25200.029.90898711
TACGGGA5300.029.73810815
ACGCAGA25300.029.6588957
CAGAGTA25450.029.61518910
GCTATCG605.642807E-428.5494913
GCAGAGT26300.028.527789
AGTACGG18200.028.0475213
GAGTACG18450.027.6674712
TGGTATC2250.027.4173132
CGGGACT500.00798862127.41404517
Produced by FastQC (version 0.11.5)