FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781725
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18290.23396974639419235No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9150.11704883430874027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA451.0654641E-437.93094
GTATCAA22100.036.046781
GTGGTAT4900.032.097011
GGTATCA10250.032.022131
CAACGCA25800.030.5652475
ATCAACG25850.030.5100293
TCAACGC25800.030.432934
AACGCAG26500.029.7578646
TGGTATC5300.029.0016272
ACGCAGA27550.028.6237147
CAGAGTA27850.028.19280610
CGCAGAG28300.027.6238828
GATTGAC500.00814207427.3102477
AGAGTAC29100.026.86446211
TATCAAC29800.026.4777742
CTCGCTA1608.913048E-1125.62466423
GCAGAGT31000.025.2179329
GACCGGA2701.1254087E-825.12335670
GAGTACG29550.023.91379512
AGTACGG29600.023.873413
Produced by FastQC (version 0.11.5)