FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085117
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGAAATTACTAAGTTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG46710.22401620628482716No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45150.21653461172682398No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41670.19984490078973988No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39940.19154800426067217No Hit
GCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC38240.18339498455002765No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37660.18061336606051362No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG37540.18003785878682108No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT37430.1795103104526029No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36630.1756735952946525No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35680.17111749604458648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.16435528557869894No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTACTTAGCCTGGTATCAGCAAAAA34170.16387569618395514No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT34020.16315631209183945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32350.15514716919961807No Hit
TTACTAAGTTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTC32130.1540920725311817No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31230.14977576797848755No Hit
GATCTATGCTGTATCCATTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA29210.14008806220466286No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28430.13634726492566124No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26350.1263718055149903No Hit
GATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAGT26110.12522079096760516No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25850.12397385854127131No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTCCCAGATCCACTGCCG24330.1166840997411656No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23590.11313513822006152No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC23240.11145657533845822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22950.11006576609370122No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22880.10973005351738055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20930.10037806031987653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103050.042.313791
GTGGTAT9050.041.913471
TCGTATG2600.038.5624140
TATCAAC114550.038.0947072
CGTATGC2650.036.578141
ATCAACG120750.036.0821083
CAACGCA123650.035.2375535
TCAACGC124100.035.1356284
AACGCAG125500.034.6636546
ACGCAGA134400.032.363567
CGCAGAG135100.032.145298
AGAGTAC136350.031.6250611
CAGAGTA138100.031.37275910
GCAGAGT143250.030.411849
TAGACCG500.00810939327.3348777
GCTAATC3700.026.91569333
ACGAGAC5150.026.5495422
TATCGTA658.6981634E-426.51819238
GGTATCA66500.026.3104041
TATACAC5900.026.0632953
Produced by FastQC (version 0.11.5)