FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085117
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGAAATTACTAAGTTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG54880.26319865983539537No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41510.1990775577581498No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38660.1854092600079516No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38110.1827715183368607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37000.17744807605520457No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAAT35660.1710215781656377No Hit
TTACTAAGTTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTC34600.16593793058135348No Hit
GCATAGATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC33710.1616695849681337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32090.15390023677328418No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT31820.15260534540747595No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30970.14852883555215365No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30240.14502783297052396No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29790.14286968069417688No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28850.1383615403835852No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28680.13754623841252073No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28260.1355319629545968No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTCCCAGATCCACTGCCG28040.13447686628616046No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTACTTAGCCTGGTATCAGCAAAAA27600.13236667294928775No Hit
GATCTATGCTGTATCCATTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA27550.13212687825191585No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT26490.1270432306676316No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25510.12234325459914239No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25490.12224733672019364No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23450.11246371306742019No Hit
GTTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTCCCAGATC22970.11016168397264998No Hit
GATCAGGAGGTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAGT22390.10738006548313597No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21650.10383110396203186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21140.1013851980488385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20930.10037806031987653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8650.043.3784031
GTATCAA99400.043.0336461
TATCAAC110150.038.893092
ATCAACG117700.036.2904783
CAACGCA120450.035.514235
TCAACGC120250.035.4600034
AACGCAG121750.035.1350176
CGCAGAG133700.032.199248
ACGCAGA132900.032.1872757
AGAGTAC135350.031.52991311
CAGAGTA137300.031.0076910
GCAGAGT141400.030.46999
GGTATCA59350.026.610761
AGTACGG98100.026.42077813
GAGTACG99300.026.2386912
GTACTAG951.1478805E-525.1345671
GAGTACT67400.024.55875412
AGAGTCG2100.024.32774711
TACGGGT36800.023.97536515
AGTACTT68250.023.95347613
Produced by FastQC (version 0.11.5)