FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821821
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.036.4854431
TCGTATG1151.8189894E-1236.09926640
GGTATCA9850.035.845411
CGTATGC1300.034.624941
CTCGTAT1450.030.98397439
ATAGCGG2250.029.94938763
AATAGCG2250.029.87978262
ATCAACG24700.029.8401133
TCTCGTA1401.4551915E-1129.59843638
TATCAAC25400.029.1527142
TCAACGC24950.029.1289124
CAACGCA25350.028.9415115
AACGCAG25550.028.44666
TAATCGT2500.027.62520638
ACGAACC2450.027.1102552
ACGCAGA28050.026.3953257
AGAGTAC27800.026.13790111
TTATACA2000.025.7110732
CGCAGAG28750.025.633438
TAGCGGC2650.025.46078564
Produced by FastQC (version 0.11.5)