FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434577
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT85180.3498759743479052No Hit
GTATATTACTGTGCGAGAAGTCGGGAGGGGAGGCAGTTACTCGTTCCCGT62320.25597875934916003No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG60890.2501050490495885No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT56610.23252499304807364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56490.23203209428167604No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56010.2300604992160856No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG55840.22936222596368896No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT55020.2259940843933053No Hit
GGTCTAGTCAGAGCCTCCTGCATTTTAATGGGAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC54920.22558333542130726No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA54300.22303669179491958No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT53900.22139369590692756No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52370.21510923663535803No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG51650.21215184403697235No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT49920.20504588682140676No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC49610.20377256500821295No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA47060.19329846622226365No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG46540.191162571567874No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT46420.1906696728014764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46360.19042322341827758No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT46220.18984817485748037No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45700.18771228020309072No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG42980.17653990816474482No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42250.17354144066915936No Hit
GGCCAAAAGTGTACGGAGTTTGTAGAGTTTGCATGCAGTAATAAACTCCA41030.16853030321078363No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39250.16121897150921904No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG38930.15990457479882542No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38900.159781350107226No Hit
GCTACATACTACGCAGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTATCTG38720.1590420019576296No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC38290.15727578137803816No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT38270.15719363158363855No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA38180.15682395750884034No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT38140.15665965792004113No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA36670.15062164803167039No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT36070.1481571541996823No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC36060.14811607930248252No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA35320.14507653690969724No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA34500.14170839533931356No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC34240.14064044801211872No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG34130.14018862414292094No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33540.13776520520813268No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGCCATGAACTGGGTCCGCCAGACTCCAAGGAAGGGG33460.13743660603053426No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA33280.13669725788093784No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC33180.1362865089089398No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA32730.13443813853494876No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32650.13410953935735037No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC32570.13378094017975198No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC32300.13267191795535735No Hit
CTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA31990.13139859614216348No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31480.12930377638497365No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT31450.12918055169337425No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT31350.12876980272137623No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACT31160.12798937967458002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31060.127578630702582No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG30880.1268392825529856No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC30600.12568918543139118No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG30420.12494983728179475No Hit
GTATGTAGCACCACCACTACCACTAATACCTGAGACCCACTCCAGCCCCTTCCTTGGAGTCTGGCGGACCCAGTT30000.12322469159940311No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29470.12104772204781364No Hit
GTCTGAGAGCCGAGGACACGGCCGTATATTACTGTGCGAGAAGTCGGGAG28920.11878860270182459No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28830.11841892862702638No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC28660.11772065537462977No Hit
AGTCAGAGCCTCCTGCATTTTAATGGGAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG28630.11759743068303036No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGA28580.11739205619703136No Hit
GTTTGTAGAGTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT28470.11694023232783354No Hit
GTTTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA27930.11472218787904429No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27910.1146400380846447No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27640.11353101586025005No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA27550.11316134178545184No Hit
GTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC27430.11266844301905422No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27300.11213446935545682No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26990.11086114754226299No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26670.10954675083186935No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA26420.10851987840187433No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26110.1072465565886805No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAACTCTACAAAC25990.10675365782228288No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAAT25760.10580893518668745No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25650.10535711131748965No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25610.10519281172869045No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25260.1037551903266974No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC25110.10313906686870039No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAACTCTACAAACT25090.10305691707430079No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA25080.10301584217710098No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA24930.10239971871910399No Hit
TTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGA24630.10116747180310993No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGA24600.10104424711151053No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24440.10038704875631373No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA24420.10030489896191414No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG24370.10009952447591512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5050.047.732911
ACTAGCG306.370981E-445.91451320
TGGTATC5350.045.6999632
GTATCAA35250.042.0068971
GGTATCA14000.041.0770951
TATCAAC40250.036.617542
CGTCTAT601.3529367E-534.46349328
TCAACGC44100.033.578324
ACCGATC2700.033.1802527
CAACGCA45150.032.9499745
AACGCAG45500.032.77226
ACGAGAC2900.032.06363322
AGTATCG1305.456968E-1231.86682334
CTTATAC3250.031.7869721
ACCCAGG26900.031.5038870
TATACAC3700.030.7130873
CGCAGAG50350.029.5457868
AGAGTAC50850.029.1186311
CAGAGTA51300.028.86438810
AATATAC20350.028.4286443
Produced by FastQC (version 0.11.5)