FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714577
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG306.5032387E-445.71903217
GTATCAA17450.038.3175281
GGTATCA7750.037.607591
TATCAAC20600.032.624752
TCAACGC20500.032.117084
ATCAACG21050.031.6015153
CAACGCA21200.031.218365
CGATAGT450.004762128530.47935518
AACGCAG22000.030.3948926
AGACGTA1600.030.01571725
TGGTATC3100.029.864852
ACGTAGA1650.029.12245827
GTATATC605.6156976E-428.5763954
CGAGACG1700.028.24612823
ACGCAGA24050.027.6634127
GGGTACG500.00796765527.431421
AGAGTAC24700.027.07237211
CGCAGAG24850.026.7728398
CAGAGTA25650.025.93781710
GTGGTAT3500.025.472031
Produced by FastQC (version 0.11.5)