FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714577
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11030.1543570531937076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21700.039.8658941
GTGGTAT3450.034.8264851
TGGTATC3550.033.8122862
GGTATCA8850.033.359221
TCAACGC25750.033.1446724
CAACGCA26050.032.8945475
ATCAACG26400.032.465263
AACGCAG26300.032.4515346
TATCAAC26600.032.232442
GTTGGTA1003.0115317E-431.77890470
ACGCAGA27950.030.5357887
AGAGTAC28550.029.77400211
CAGAGTA29350.029.0792310
CGCAGAG29550.028.8824128
GTATAAG2050.028.4679241
GCAGAGT30550.027.9369989
TAATAGT1058.12548E-726.1151374
GAGTACG23100.025.66999412
AGTACGG23000.025.63257613
GTATATG1101.2027267E-624.966521
Produced by FastQC (version 0.11.5)