FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959291
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT90060.45965606946594456No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC78340.3998385130131257No Hit
GTATAATAGTTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT78320.39973643527173863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62780.3204220302139907No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC59320.30276258095402875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58100.2965358387294179No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57970.29587233341040203No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57130.29158506827214536No Hit
GTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT57120.29153402940145184No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG56710.28944143570301706No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53100.2710164033826522No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50790.2592264242524464No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACA47120.24049515870792038No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG46780.2387598371043403No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45330.23135920085377823No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAA44160.22538765298263505No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT42210.21543507319739638No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41850.21359767385242928No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41390.21124988580052684No Hit
TCCATAGGGCGTCTAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT41150.21002495290388207No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG40890.20869794226585026No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37230.19001771559201772No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCCGATGATTTTGCAACTTA37190.1898135601092436No Hit
CAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATAGGGCGT36870.1881803162470506No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT35610.1817494185396656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35580.18159630192758502No Hit
CCCTATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTACTAATACTGTGA34250.17480813212534535No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33960.17332800487523292No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG33510.171031255694024No Hit
ATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA33360.17026567263362105No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC31610.16133387026225302No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTGTAACTATTATA31510.16082348155531773No Hit
GATCCATAGGGCGTCTAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA31030.15837361576202819No Hit
CAGTATAATAGTTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACG30790.15714868286538344No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30710.1567403718998352No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30620.15628102206359343No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30560.15597478883943222No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30450.15541336126180338No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGGCTGC30060.15342284530475564No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30040.1533207675633686No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29510.15061570741661143No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28880.14740025856291894No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28490.1454097426058712No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28400.1449503927696294No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27940.14260260471772696No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC27520.14045897214859865No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27500.14035689440721158No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26660.13606962926895494No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT26330.1343853465360684No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG25770.13152716977723064No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACAGTATTAGTAA25230.12877107075977995No Hit
GTCACAGTATTAGTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATAGGG24010.1225443285351691No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23970.12234017305239497No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23930.12213601756962085No Hit
CACCATCAGCAGCCTGCAGCCCGATGATTTTGCAACTTATTACTGCCAAC23750.12121731789713729No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATAGGGCGTCTAGTTTACAAAGTGGGGTCCCAT23710.12101316241436315No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22880.11677693614680004No Hit
CCTTGGTCCCCTGGCCAAAAGTGTAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCGGGCTGCAGG22360.1141229148707364No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22180.11320421519825283No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21940.11197928230160807No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21930.11192824343091456No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21930.11192824343091456No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC21510.10978461086178623No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21510.10978461086178623No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21480.10963149424970563No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21420.10932526102554443No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21350.10896798893068974No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCCGATGATTTTG20930.1068243563615614No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20400.10411929621480423No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20160.10289436331815949No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19900.10156735268012766No Hit
CTATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC19880.10146527493874058No Hit
ACCTTGATGGGACCCCACTTTGTAAACTAGACGCCCTATGGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG19850.10131215832666No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19780.10095488623180528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4850.040.390131
GTATCAA36800.039.0364721
CTACGCT2050.038.59655828
ACTACGC2200.034.39161327
TATCAAC42400.034.2049332
CGAGACT2300.032.8845723
ATCAACG44400.032.5875973
TCAACGC44350.032.5468444
CAACGCA44950.032.34265
AACGCAG45950.031.7891436
ACGAGAC2500.030.2491722
GTAGTTT2651.0913936E-1130.20965670
TATACAC3300.030.202113
ACGCAGA49800.029.3285527
CGCAGAG50800.028.683578
CCTTTGT8850.028.64512470
TACGCTG2650.028.56466529
AGAGTAC51800.027.93008611
GTACTAG756.329978E-527.4936331
CAGAGTA53100.027.246310
Produced by FastQC (version 0.11.5)