FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241002
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34540.27832348376553784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29350.23650243915803523No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26880.21659916744695015No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25210.20314229952892904No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22260.17937118554200557No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21370.1721995613222219No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21180.1706685404213692No Hit
CTATAATATTGTTGGCAGTAATAAGTAGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC21120.1701850601368894No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20680.1666395380507042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20430.1646250368653717No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19960.1608377746369466No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19450.1567281922188683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19290.1554389114602555No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACTTTGGTCCCAGGGCCGAAACCA18710.15076526871028412No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17810.14351306444308712No Hit
GAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGATAGCCCCTAAGCTCCTAATCAATAAGGCATC17670.14238494377930092No Hit
GTATTGGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGATAGCCCCT17410.14028986254655512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17390.14012870245172854No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG17360.13988696230948863No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17160.13827536136122262No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16900.13618028012847683No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16490.13287649818453154No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16180.13037851671471923No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16170.13029793666730594No Hit
ATATTATAGTGATTCTCGTGGTTTCGGCCCTGGGACCAAAGTGGACATCAAACGAACTGTGGCTGCACCAT15890.12804169533973353No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15280.12312631244752224No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15120.12183703168890944No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15000.12087007111994984No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCTACTTA14580.11748570912859124No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14170.11418192718464595No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14110.11369844690016614No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13960.11248974618896666No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13670.11015292481398097No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13360.10765494334416867No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA13280.10701030296486227No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13240.10668798277520905No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13090.10547928206400957No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13040.10507638182694307No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12840.10346478087867707No Hit
GTAATAAGTAGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12760.10282014049937066No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAAA12570.10128911959851797No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12570.10128911959851797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1950.040.83064340
GTATCAA22150.039.730041
GTGGTAT3550.038.582581
CGAGACG2500.035.6404523
CTCGTAT2450.033.8818339
CGTATGC2500.033.25833541
TATCAAC26650.033.0227362
ATCAACG27200.032.3549963
TCAACGC27250.032.295634
ACGAGAC2800.031.8141322
CAACGCA27800.031.7798675
AACGCAG28400.031.108466
AGAGTAC30950.028.42903311
CGCAGAG31100.028.4031498
ATACACA3550.027.97354
TACACAT3350.027.5991765
TATACAC3500.027.3947373
GCAGAGT32800.026.9299499
GGAGCTA3350.026.64462929
CTTATAC3350.026.5759141
Produced by FastQC (version 0.11.5)