FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241002
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32570.26244921442511776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27470.2213533902443348No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23150.18654280976178927No Hit
CTATAATATTGTTGGCAGTAATAAGTAGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC22830.18396424824456367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22040.17759842449891297No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21960.17695378411960658No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21880.17630914374030016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21780.17550334326616718No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21700.17485870288686078No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21610.17413348246014107No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19940.16067661454211998No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACTTTGGTCCCAGGGCCGAAACCA19770.1593067537360939No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19450.1567281922188683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19000.15310209008526982No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18490.14899250766719152No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18230.14689742643444573No Hit
ATATTATAGTGATTCTCGTGGTTTCGGCCCTGGGACCAAAGTGGACATCAAACGAACTGTGGCTGCACCAT17670.14238494377930092No Hit
GAGTATTGGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGATAGCCCCTAAGCTCCTAATCAATAAGGCATC16450.1325541779948783No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16190.13045909676213252No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15950.12852517562421334No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15870.12788053524490695No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG15300.12328747254234886No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCTACTTA15190.12240109202080254No Hit
GTATTGGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGATAGCCCCT14830.11950021031392376No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14660.11813034950789766No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14660.11813034950789766No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14240.11474598751653904No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14180.11426250723205926No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14060.11329554666309966No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13990.11273148633120657No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13810.11128104547776715No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13610.10966944452950117No Hit
GTAATAAGTAGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13600.10958886448208788No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13350.10757436329675536No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13140.10588218230107606No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA12900.10394826116315688No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12590.10145027969334457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4500.037.32381
GTATCAA24500.037.0750731
TCGGTAA501.7426703E-434.88937450
TAGGTCG400.00269946134.2272345
TATCAAC27750.033.1908722
ATCAACG29200.031.5325243
TCAACGC29400.031.3167554
CCGTTAA553.4107378E-431.11817616
CAACGCA29500.030.978555
AACGCAG29650.030.9372656
GCATACG450.004771840830.4684051
AGAGTAC32000.028.12937711
TCCTATA1950.028.0838854
CGGTAAA500.007303757627.92640951
CGCAGAG32950.027.631098
TTCGGGG7450.027.3473870
TAACGGC906.928938E-626.82220536
ACGCAGA34900.026.3814497
GCAGAGT34650.026.3742319
CCTATAG2100.026.077895
Produced by FastQC (version 0.11.5)