FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715520
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19390.27099172629695883No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19100.2669387298747764No Hit
GTGTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18390.2570158765652952No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14290.19971489266547407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14090.1969197227191413No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13990.19552213774597496No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13850.19356551878354206No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13630.19049083184257604No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13050.1823848389982111No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13000.1816860465116279No Hit
GTAGTACACTGCACAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12380.17302101967799643No Hit
GTGTATTATTGTGCGAGGGACATAGGATATTGTAGTGATCCCAGTTGCTATGCGGACTGGTTCGACCCCTGGGGC12210.17064512522361358No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10810.15107893559928443No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10720.14982110912343471No Hit
GTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCACCTGTG10670.14912231663685152No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10530.1471656976744186No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10530.1471656976744186No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10220.14283318425760286No Hit
CTATATGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGCACTCACTGTATCTGCAAATGAA10220.14283318425760286No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9770.1365440518783542No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9760.13640429338103754No Hit
GTATTATTGTGCGAGGGACATAGGATATTGTAGTGATCCCAGTTGCTATG9410.13151274597495527No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9270.12955612701252237No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9230.12899709302325582No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9220.12885733452593917No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9200.1285778175313059No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9120.1274597495527728No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT8920.12466457960644008No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA8890.12424530411449017No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8710.1217296511627907No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA8670.12117061717352416No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8610.12033206618962432No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8480.11851520572450805No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA8380.11711762075134168No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8250.11530076028622539No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8250.11530076028622539No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8220.11488148479427548No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8150.11390317531305905No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA7920.11068872987477639No Hit
CTCCAGAGACAACGCCAAGCACTCACTGTATCTGCAAATGAACAGCCTGA7800.10901162790697674No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC7420.10370080500894455No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT7310.10216346153846155No Hit
GTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGA7300.1020237030411449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA200.007496442651.50640525
GTATCAA13350.040.893611
TCAACGC14800.036.6039164
ATCAACG14900.036.3633353
TATCAAC15050.036.0311282
CAACGCA15200.035.640655
GGTATCA4800.035.0505181
AACGCAG15500.034.508426
TGGTATC2400.034.320792
GTGGTAT2550.033.661961
CGCAGAG16150.032.907238
CGAACTA851.2460805E-732.3539729
AGAGTAC16300.031.9733511
TAACCGT652.4205317E-531.6497345
GCAGAGT16700.031.4128349
GTTAGGC450.00476027730.4817813
GTCTGAT703.8896824E-430.42488969
CAGAGTA17150.030.38866610
ATAGCGG604.7606038E-429.39432163
CTAACCG704.0149484E-529.389044
Produced by FastQC (version 0.11.5)