FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495373
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAG87260.5835333391735708No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC69050.4617577019245365No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATT64950.4343397934829638No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51500.3443956792051214No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48720.3258049998227867No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTG45540.3045394025437132No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43250.28922549758488353No Hit
CACTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGCAACACGGTCTCCCTGAAAATCT42670.28534686663461223No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC41580.278057715365999No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGGAGA40080.26802677325322843No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG39980.26735804377904376No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37780.2526459953469803No Hit
ACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC35350.23639586912429209No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAAT35090.23465717249141183No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34960.23378782417497174No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34130.22823736953923873No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTT33710.22542870574766297No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33300.22268691490350567No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31520.21078353026301797No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30170.20175568236152452No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28700.19192535909100938No Hit
CCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCG28380.18978542477361837No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28160.188314219930412No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27820.18604053971818402No Hit
GATTATTACTGCTGCTCACATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGT27440.18349936771628214No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27100.18122568750405416No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26690.1784838966598969No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA26520.1773470565537829No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26320.17600959760541351No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26270.17567523286832115No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCACTAC26170.17500650339413645No Hit
GTCGATCACCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATG25690.17179660191804988No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25180.16838608159970792No Hit
CTAAGAGGCCCTCAGGGATTTCTAATCGGTTCTCTGGTTCCAAGTCTGGC24910.1665805120194092No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24620.16464119654427356No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC24100.16116380327851312No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC23880.15969259843530678No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23700.1584888853817743No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23570.15761953706533421No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23440.1567501887488941No Hit
CATGCAGGTAGTGGGTCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC23240.15541272980052467No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22570.15093224232348718No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22380.14966165632253625No Hit
TTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTCTCCTGGTACCAGCAACATCCAGGCAAAGC21650.14477993116098792No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21440.14337559926520005No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21410.14317498042294463No Hit
CTCTTAGTGTCCTCATAAATTATGAGTCGGGGGGCTTTGCCTGGATGTTG20860.13949696831492878No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20720.13856074705107022No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20570.13755765283979315No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC20310.13581895620691292No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19580.1309372310453646No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19560.13080348515052767No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19430.12993413683408755No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19090.12766045662185957No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG18800.12572114114672392No Hit
GCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATTTTCAGGG18760.12545364935705006No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGACCCA18740.1253199034622131No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18670.12485179283028382No Hit
CCATTTCCTGCACTGGAACCAGCAATGATATTGGGAATTATGACCTTGTC18550.12404931746126217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.11923446524713233No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCA17520.11716140387715976No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGACCCACTACCTGCATGTGAGCAGCAGTAATAATCAGCCTCGTCGTCAG17040.11395150240107318No Hit
GTACCAGCAACATCCAGGCAAAGCCCCCCGACTCATAATTTATGAGGACA17040.11395150240107318No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16960.11341651882172542No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC16850.11268091640012225No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16750.11201218692593753No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC16400.10967163376629108No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16360.10940414197661721No Hit
GTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAG16290.1089360313446879No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15760.10539176513150898No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15560.1040543061831396No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT15440.10325183081411796No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC15430.10318495786669948No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15280.10218186365542244No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15180.10151313418123772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.043.924571
GTATCAA33650.043.3604431
ACGCTAG350.001365379339.32564517
TATCAAC37950.038.084692
TCAACGC40450.035.9034274
AAGTCCG951.5263117E-435.68288870
ATCAACG40650.035.6409263
CAACGCA41450.035.286325
ACTCGAC2050.035.2775127
CGAGACT2100.034.4179923
AACGCAG42650.034.2935036
TCGTATG751.6290596E-632.30171240
ACGCAGA46250.031.5476537
AGAGTAC46500.031.22794311
CGCAGAG47050.030.93818
GACTCGA2350.030.76988426
TCGACGT2150.030.44237329
CAGAGTA48000.030.39646110
ACGAGAC2400.030.11473822
GTGGTAT4950.029.8934611
Produced by FastQC (version 0.11.5)